WWTR1/TAZ Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

WWTR1/TAZ Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(69)

Rezensionen (2)

Kat-Nr. : 23306-1-AP

Synonyme

TAZ, WWTR1, WW domain-containing transcription regulator protein 1, Transcriptional coactivator with PDZ-binding motif

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  WB analysis using 23306-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23306-1-AP (TAZ antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 23306-1-AP

  WB result of TAZ antibody (23306-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TAZ transfected HeLa cells.

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  WB analysis of LN229 cells using 23306-1-AP

  WB result of WWTR1 antibody (TAZ antibody, 23306-1-AP, 1:1000) with LN229 cell lysate by Dr. Xiaobing He. (Lane 1, LN229 (GBM cell line) cell lysate. 90 ug; Lane 2, LN229 growth inhibition treatment. 90 ug).

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  WB analysis of MDA-MB-453s using 23306-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 23306-1-AP (WWTR1,TAZ Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of MDA-MB-453s using 23306-1-AP

  IP result of anti-WWTR1 (IP:23306-1-AP, 4ug; Detection:23306-1-AP 1:300) with MDA-MB-453s cells lysate 2800ug.

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  IP experiment of MDA-MB-453s using 23306-1-AP

  IP result of anti-WWTR1 (IP:23306-1-AP, 4ug; Detection:23306-1-AP 1:5000) with MDA-MB-453s cells lysate 2800 ug.

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  IHC staining of human ovary cancer using 23306-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 23306-1-AP (WWTR1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat kidney using 23306-1-AP

  Immunofluorescent analysis of (4% PFA) fixed rat kidney tissue using WWTR1 antibody (23306-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of U-251 using 23306-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using WWTR1 antibody (23306-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, LN229-Zellen, MDA-MB-453s-Zellen
• Erfolgreiche IP: MDA-MB-453s-Zellen
• Erfolgreiche Detektion in IHC: human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Rattennierengewebe
• Erfolgreiche Detektion in IF/ICC: U-251-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 8 publications below
• WB: See 59 publications below
• IHC: See 14 publications below
• IF: See 11 publications below
• IP: See 4 publications below
• CoIP: See 2 publications below
• ChIP: See 1 publications below

Produktinformation

23306-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA WWTR1/TAZ und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: WWTR1/TAZ fusion protein Ag19659
• Vollständiger Name: WW domain containing transcription regulator 1
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC014052
• Gene symbol: WWTR1
• Gene ID (NCBI): 25937
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

TAZ, also referred as WWTR1, is a transcriptional co-activator with a PDZ-binding motif that is regulated by its interaction with 14-3-3 protein. TAZ is expressed in many primary tumors, such as breast cancer, thyroid carcinoma, colorectal cancer, and glioma (PMID: 22003437, PMID: 27764783). TAZ has been reported to be one of the nuclear effectors of Hippo-related pathways that regulate organ size control, cancer stem cell (CSC) properties, and epithelial-mesenchymal transition (EMT). TAZ has been defined for its role in the nucleus, where it functions directly as a transcriptional regulator by interacting with several nuclear factors as Runx2/Cbfa1, NHERF-2, TEF-1, TBX5, PAX3, PAX8 and TTF-1.

Publikationen

Species	Application	Title
human,mouse	IF,IP	Cancer Cell; Programming of Schwann Cells by Lats1/2-TAZ/YAP Signaling Drives Malignant Peripheral Nerve Sheath Tumorigenesis.; Authors - Lai Man Natalie Wu
human	WB,IHC	Nat Neurosci; YAP and TAZ control peripheral myelination and the expression of laminin receptors in Schwann cells.; Authors - Yannick Poitelon ; KO Validated
human	WB	Adv Sci (Weinh); DNMT3A Cooperates with YAP/TAZ to Drive Gallbladder Cancer Metastasis; Authors - Sunwang Xu
mouse,human	WB	Sci Adv; YAP drives fate conversion and chemoresistance of small cell lung cancer.; Authors - Qingzhe Wu ; KD Validated
mouse	WB,IF	Bone Res; TAZ inhibits osteoclastogenesis by attenuating TAK1/NF-κB signaling.; Authors - Wanlei Yang ; KO Validated
human	WB	Clin Transl Med; NSUN2 promotes colorectal cancer progression by enhancing SKIL mRNA stabilization; Authors - Shaomin Zou

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Yohanes (Verified Customer) (04-24-2023)

• Applications: Western Blot, Immunofluorescence, Other
• Primary Antibody Dilution: 1:1000 for WB, 1:200 for IF, 1:100 for PLA
• Cell Tissue Type: bEnd.3 mouse Endothelial cell

FH

Joshua (Verified Customer) (02-28-2020)

MDCKs fixed in 4% paraformaldehyde. Bright Mix of nuclear and cytosolic staining

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: MDCK

WWTR1/TAZ Antibody Immunofluorescence, validation (1:500 dilution) in MDCK (Cat no:23306-1-AP)