XAB2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

XAB2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Rezensionen (2)

Kat-Nr. : 10637-1-AP

Synonyme

Pre-mRNA-splicing factor SYF1, PP3898, NTC90, KIAA1177, HCRN

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Galerie der Validierungsdaten

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  WB analysis of K-562 using 10637-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10637-1-AP (XAB2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10637-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10637-1-AP (XAB2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10637-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10637-1-AP (XAB2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 10637-1-AP

  IP result of anti-XAB2 (IP:10637-1-AP, 4ug; Detection:10637-1-AP 1:1000) with K-562 cells lysate 1600 ug.

• 

  IHC staining of mouse salivary gland using 10637-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse salivary gland tissue slide using 10637-1-AP (XAB2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse salivary gland using 10637-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse salivary gland tissue slide using 10637-1-AP (XAB2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat salivary gland using 10637-1-AP

  Immunohistochemical analysis of paraffin-embedded rat salivary gland tissue slide using 10637-1-AP (XAB2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat salivary gland using 10637-1-AP

  Immunohistochemical analysis of paraffin-embedded rat salivary gland tissue slide using 10637-1-AP (XAB2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 10637-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 10637-1-AP (XAB2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 10637-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 10637-1-AP (XAB2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 10637-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10637-1-AP (XAB2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 10637-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10637-1-AP (XAB2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of U2OS using 10637-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using XAB2 antibody (10637-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: rat salivary gland tissue, Maushodengewebe, Rattenhodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

10637-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA XAB2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: XAB2 fusion protein Ag0999
• Vollständiger Name: XPA binding protein 2
• Berechnetes Molekulargewicht: 100 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC007208
• Gene symbol: XAB2
• Gene ID (NCBI): 56949
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

XPA-binding protein 2 (XAB2) a tetratricopeptide repeat protein involved in transcription-coupled DNA repair and transcription. XAB2 is involved in pre-mRNA splicing, transcription, and transcription-coupled DNA repair, leading to preimplantation lethality, and is essential for mouse embryogenesis. It interactes with Cockayne syndrome groups A and B (CSA and CSB) proteins and RNA polymerase II, which are known to be involved in transcription-coupled repair (TCR) and transcription, and that the antibodies against XAB2 protein inhibited the recovery of RNA synthesis after UV irradiation and normal RNA synthesis when microinjected into living fibroblasts.

Publikationen

Species	Application	Title
human	WB	Nucleic Acids Res; XAB2 depletion induces intron retention in POLR2A to impair global transcription and promote cellular senescence.; Authors - Shuai Hou ; KD Validated
human	WB	Nucleic Acids Res; Evidence that a consensus element found in naturally intronless mRNAs promotes mRNA export.; Authors - Lei Haixin H ; KD Validated
human	WB,IF	Cell Death Dis; XAB2 functions in mitotic cell cycle progression via transcriptional regulation of CENPE.; Authors - Shuai Hou ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Moritz (Verified Customer) (08-19-2024)

Antibody worked well for WB, showing a band at ~ 100kDa using HEK293T WT cell lysate, a dilution of 1:3000 and 1h incubation time at RT.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: HEK293T WT lysate

FH

Tsimafei (Verified Customer) (08-12-2024)

Antibody detect a strong band ~ 100 kDa

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: mESCs

XAB2 Antibody Western Blot validation (1:3000 dilution) in mESCs (Cat no:10637-1-AP)