XAB2 Polyklonaler Antikörper

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• KD/KO Validated

XAB2 Polyklonal Antikörper für IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 10637-1-AP

Synonyme

HCNP, HCRN, KIAA1177, NTC90, Pre mRNA splicing factor SYF1, Protein HCNP, SYF1, XAB2, XPA binding protein 2

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Galerie der Validierungsdaten

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  WB analysis of K-562 using 10637-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10637-1-AP (XAB2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10637-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10637-1-AP (XAB2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10637-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10637-1-AP (XAB2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 10637-1-AP

  IP Result of anti-XAB2 (IP:10637-1-AP, 4ug; Detection:10637-1-AP 1:200) with K-562 cells lysate 3200ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HeLa-Zellen
• Erfolgreiche IP: K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

10637-1-AP bindet in WB, IP, IF, ELISA XAB2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: XAB2 fusion protein Ag0999
• Vollständiger Name: XPA binding protein 2
• Berechnetes Molekulargewicht: 100 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC007208
• Gene symbol: XAB2
• Gene ID (NCBI): 56949
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

XPA-binding protein 2 (XAB2) a tetratricopeptide repeat protein involved in transcription-coupled DNA repair and transcription. XAB2 is involved in pre-mRNA splicing, transcription, and transcription-coupled DNA repair, leading to preimplantation lethality, and is essential for mouse embryogenesis. It interactes with Cockayne syndrome groups A and B (CSA and CSB) proteins and RNA polymerase II, which are known to be involved in transcription-coupled repair (TCR) and transcription, and that the antibodies against XAB2 protein inhibited the recovery of RNA synthesis after UV irradiation and normal RNA synthesis when microinjected into living fibroblasts.

Publikationen

Species	Application	Title
human	WB	Nucleic Acids Res; XAB2 depletion induces intron retention in POLR2A to impair global transcription and promote cellular senescence.; Authors - Shuai Hou ; KD Validated
human	WB	Nucleic Acids Res; Evidence that a consensus element found in naturally intronless mRNAs promotes mRNA export.; Authors - Lei Haixin H ; KD Validated
human	WB,IF	Cell Death Dis; XAB2 functions in mitotic cell cycle progression via transcriptional regulation of CENPE.; Authors - Shuai Hou ; KD Validated