XBP1S-specific Polyklonaler Antikörper

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• KD/KO Validated

XBP1S-specific Polyklonal Antikörper für WB, IHC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, FC (Intra), ELISA

Konjugation

Unkonjugiert

Publikationen(110)

Rezensionen (1)

Kat-Nr. : 24868-1-AP

Synonyme

XBP1, Tax-responsive element-binding protein 5, TREB-5, X box binding protein 1, X-box-binding protein 1

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  WB analysis using 24868-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24868-1-AP (XBP1S-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24868-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24868-1-AP (XBP1S-specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 24868-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 24868-1-AP (XBP1S-specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24868-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24868-1-AP (XBP1S-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 24868-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24868-1-AP (XBP1S antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 24868-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24868-1-AP (XBP1S-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 24868-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24868-1-AP (XBP1S antibody at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 24868-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24868-1-AP (XBP1S antibody at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 24868-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24868-1-AP (XBP1S antibody at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 24868-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24868-1-AP (XBP1S antibody at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 24868-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 24868-1-AP (XBP1S antibody at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 24868-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 24868-1-AP (XBP1S antibody at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 24868-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24868-1-AP (XBP1S antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  FC experiment of HepG2 using 24868-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human XBP1S-specific (24868-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 90% MeOH and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, MCF-7-Zellen, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Leberkarzinomgewebe, Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 102 publications below
• IHC: See 10 publications below
• IF: See 13 publications below

Produktinformation

24868-1-AP bindet in WB, IHC, IF, FC (Intra), ELISA XBP1S-specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: XBP1S-specific fusion protein Ag21703
• Vollständiger Name: X-box binding protein 1
• Berechnetes Molekulargewicht: 261 aa, 29 kDa
• Beobachtetes Molekulargewicht: 45-65 kDa
• GenBank-Zugangsnummer: BC000938
• Gene symbol: XBP1
• Gene ID (NCBI): 7494
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

XBP1, also named as XBP2, belongs to the bZIP family. The X-box-binding protein-1 (XBP1) is a transcriptional regulator of the ER stress response that lies downstream of inositol-requiring enzyme 1 (IRE1α) activation (PMID: 14559994). XBP1 localizes in the nucleus. As a transcription factor, it is essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response. XBP1 has an association with major affective disorder. The unspliced XBP1U isoform is composed of 261 amino acid residues, and the spliced XBP1S isoform is composed of 376 amino acid residues. The calculated molecular weight of protein generated from the unspliced XBP1 isoform XBP1U mRNA is 33 kDa, and protein generated from spliced XBP1 isoform XBP1S mRNA is 40 kDa, and shows at 45-65 kDa range. This antibody specially recognizes the XBP1S isoform.

Publikationen

Species	Application	Title
mouse	WB,IF	Nat Commun; A nanoemulsion targeting adipose hypertrophy and hyperplasia shows anti-obesity efficiency in female mice; Authors - Yichao Lu
mouse	IF	Acta Pharm Sin B; The substitution of SERCA2 redox cysteine 674 promotes pulmonary vascular remodeling by activating IRE1α/XBP1s pathway.; Authors - Weimin Yu
mouse	WB	Nat Commun; Activation of GCN2/ATF4 signals in amygdalar PKC-δ neurons promotes WAT browning under leucine deprivation.; Authors - Feixiang Yuan
human	WB	Cancer Lett; LIMK1 m6A-RNA methylation recognized by YTHDC2 induces 5-FU chemoresistance in colorectal cancer via endoplasmic reticulum stress and stress granule formation; Authors - Lu Chen
	WB	Diabetes; Lipid Droplets Protect Human β-Cells From Lipotoxicity-Induced Stress and Cell Identity Changes.; Authors - Xin Tong
human	WB	Oxid Med Cell Longev; Drp1-Mediated Mitochondrial Metabolic Dysfunction Inhibits the Tumor Growth of Pituitary Adenomas.; Authors - Kexia Fan

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kevin (Verified Customer) (08-19-2019)

Good antibody. Clear and sharp bands on liver tissue from mouse.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Mouse liver tissue