XBP1S-specific Rekombinanter Antikörper

XBP1S-specific Rekombinant Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241004C10

Kat-Nr. : 83959-6-RR

Synonyme

XBP1, 241004C10, Tax-responsive element-binding protein 5, TREB-5, X box binding protein 1

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  IHC staining of mouse liver using 83959-6-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83959-6-RR (XBP1S-specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 83959-6-RR

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83959-6-RR (XBP1S-specific antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83959-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83959-6-RR against Human XBP1S-specific were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: Mauslebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83959-6-RR bindet in IHC, ELISA XBP1S-specific und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: XBP1S-specific fusion protein Ag21703
• Vollständiger Name: X-box binding protein 1
• Berechnetes Molekulargewicht: 261 aa, 29 kDa
• GenBank-Zugangsnummer: BC000938
• Gene symbol: XBP1
• Gene ID (NCBI): 7494
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

XBP1, also named as XBP2, belongs to the bZIP family. The X-box-binding protein-1 (XBP1) is a transcriptional regulator of the ER stress response that lies downstream of inositol-requiring enzyme 1 (IRE1α) activation (PMID: 14559994). XBP1 localizes in the nucleus. As a transcription factor, it is essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response. XBP1 has an association with major affective disorder. The unspliced XBP1U isoform is composed of 261 amino acid residues, and the spliced XBP1S isoform is composed of 376 amino acid residues.