XIAP Polyklonaler Antikörper

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• KD/KO Validated

XIAP Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(51)

Kat-Nr. : 10037-1-Ig

Synonyme

API3, Baculoviral IAP repeat-containing protein 4, E3 ubiquitin-protein ligase XIAP, EC:2.3.2.27, hIAP-3

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  WB analysis using 10037-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 10037-1-Ig (XIAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10037-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10037-1-Ig (XIAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 10037-1-Ig

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10037-1-Ig (XIAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10037-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10037-1-Ig (XIAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10037-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 10037-1-Ig (XIAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 10037-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10037-1-Ig(XIAP antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: RAW 264.7-Zellen, C6-Zellen, COLO 320-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, NIH/3T3-Zellen, Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 45 publications below
• IHC: See 11 publications below
• CoIP: See 1 publications below

Produktinformation

10037-1-Ig bindet in WB, IHC, CoIP, ELISA XIAP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: XIAP fusion protein Ag0022
• Vollständiger Name: X-linked inhibitor of apoptosis
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 45-57 kDa
• GenBank-Zugangsnummer: BC032729
• Gene symbol: XIAP
• Gene ID (NCBI): 331
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

XIAP, also named as API3, BIRC4 and IAP3, belongs to the IAP family. It has E3 ubiquitin-protein ligase activity. It mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. XIAP is an inhibitor of caspase-3, -7 and -9. It mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B. XIAP is an apoptotic suppressor. It is ubiquitinated and degraded by the proteasome in apoptotic cells. The MW of XIAP is 45-56 kDa.

Publikationen

Species	Application	Title
human	WB	Nat Commun; Targeting dual-specificity tyrosine phosphorylation-regulated kinase 2 with a highly selective inhibitor for the treatment of prostate cancer.; Authors - Kai Yuan
human	WB	Cancer Res; DAB2IP is a bifunctional tumor suppressor that regulates wildtype RAS and inflammatory cascades in KRAS mutant colon cancer; Authors - Abigail L Miller
human	IHC	Cancer Res; An ATM/TRIM37/NEMO axis counteracts genotoxicity by activating nuclear-to-cytoplasmic NF-κB signaling.; Authors - Geyan Wu
human	WB	EMBO Rep; XIAP-mediated degradation of IFT88 disrupts HSC cilia to stimulate HSC activation and liver fibrosis; Authors - Renjie Hong ; KD Validated
human	WB	Acta Pharmacol Sin; Aspirin modulates succinylation of PGAM1K99 to restrict the glycolysis through NF-κB/HAT1/PGAM1 signaling in liver cancer.; Authors - Yu-Fei Wang
human	WB	Int J Nanomedicine; Synergism of cisplatin-oleanolic acid co-loaded calcium carbonate nanoparticles on hepatocellular carcinoma cells for enhanced apoptosis and reduced hepatotoxicity.; Authors - Muhammad Waseem Khan

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