XIAP Polyklonaler Antikörper

XIAP Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 23453-1-AP

Synonyme

IAP-3, hIAP-3, EC:2.3.2.27, E3 ubiquitin-protein ligase XIAP, Baculoviral IAP repeat-containing protein 4

---

• 

  WB analysis using 23453-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23453-1-AP (XIAP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 23453-1-AP

  Tunicamycin treated HepG2 cells were subjected to SDS PAGE followed by western blot with 23453-1-AP (XIAP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 23453-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 23453-1-AP (XIAP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon using 23453-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 23453-1-AP (XIAP antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon using 23453-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 23453-1-AP (XIAP antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 23453-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 23453-1-AP (XIAP antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 23453-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23453-1-AP (XIAP antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 23453-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 23453-1-AP (XIAP antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 23453-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 23453-1-AP (XIAP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, C6-Zellen, HEK-293-Zellen, NIH/3T3-Zellen, mit Tunicamycin behandelte HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Nierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

23453-1-AP bindet in WB, IHC, IF/ICC, ELISA XIAP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: XIAP fusion protein Ag0725
• Vollständiger Name: X-linked inhibitor of apoptosis
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC032729
• Gene symbol: XIAP
• Gene ID (NCBI): 331
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

XIAP, also named as API3, BIRC4 and IAP3, belongs to the IAP family. It has E3 ubiquitin-protein ligase activity. It mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. XIAP is an inhibitor of caspase-3, -7 and -9. It mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B. XIAP is an apoptotic suppressor. It is ubiquitinated and degraded by the proteasome in apoptotic cells. The MW of XIAP is 45-56kd.

Publikationen

Species	Application	Title
human	WB,IHC	Cancer Lett; Esculetin inhibits endometrial cancer proliferation and promotes apoptosis via hnRNPA1 to downregulate BCLXL and XIAP.; Authors - Ruqi Jiang
human	WB,IF	Biochim Biophys Acta Mol Cell Res; HTRA3 transcriptionally inhibited by FOXP1 suppresses tumorigenesis of osteosarcoma via the PTEN/PI3K/AKT pathway; Authors - Ziyuan Tong
human	WB	Front Immunol; Metabolic Reprogramming Mediates Delayed Apoptosis of Human Neutrophils Infected With Francisella tularensis.; Authors - Samantha J Krysa
human	WB	Environ Pollut; DBDPE upregulates NOD-like receptor signaling to induce NLRP3 inflammasome-mediated HAECs pyroptosis; Authors - Li Jing