XIAP Monoklonaler Antikörper

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• KD/KO Validated

XIAP Monoklonal Antikörper für WB, IHC, IF-P, FC (Intra), Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2A1A12

Kat-Nr. : 66800-1-PBS

Synonyme

API3, BIRC4, hIAP 3, hIAP3, hILP, IAP 3, IAP like protein, IAP3, ILP, ILP1, MIHA, X linked IAP, XIAP, XLP2

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  WB analysis using 66800-1-Ig (same clone as 66800-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66800-1-Ig (XIAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66800-1-Ig (same clone as 66800-1-PBS)

  WB result of XIAP antibody (66800-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-XIAP transfected HEK-293 cells. This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

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  WB analysis using 66800-1-Ig (same clone as 66800-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66800-1-Ig (XIAP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66800-1-Ig (same clone as 66800-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66800-1-Ig (XIAP antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66800-1-Ig (same clone as 66800-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66800-1-Ig (XIAP antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66800-1-Ig (same clone as 66800-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66800-1-Ig (XIAP antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66800-1-Ig (same clone as 66800-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66800-1-Ig (XIAP antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66800-1-Ig (same clone as 66800-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66800-1-Ig (XIAP antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66800-1-Ig (same clone as 66800-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66800-1-Ig (XIAP antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

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  IF Staining of human colon cancer using 66800-1-Ig (same clone as 66800-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using XIAP antibody (66800-1-Ig, Clone: 2A1A12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

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  IF Staining of human colon using 66800-1-Ig (same clone as 66800-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using XIAP antibody (66800-1-Ig, Clone: 2A1A12 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human colon using 66800-1-Ig (same clone as 66800-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using XIAP antibody (66800-1-Ig, Clone: 2A1A12 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 66800-1-Ig (same clone as 66800-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human XIAP (66800-1-Ig, Clone:2A1A12) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66800-1-PBS bindet in WB, IHC, IF-P, FC (Intra), Indirect ELISA XIAP und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: XIAP fusion protein Ag18088
• Vollständiger Name: X-linked inhibitor of apoptosis
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 57 kDa
• GenBank-Zugangsnummer: BC032729
• Gene symbol: XIAP
• Gene ID (NCBI): 331
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

XIAP, also named as API3, BIRC4 and IAP3, belongs to the IAP family. It has E3 ubiquitin-protein ligase activity. It mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. XIAP is an inhibitor of caspase-3, -7 and -9. It mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B. XIAP is an apoptotic suppressor. It is ubiquitinated and degraded by the proteasome in apoptotic cells.