XIRP2 Polyklonaler Antikörper

XIRP2 Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 11896-1-AP

Synonyme

Xin actin-binding repeat-containing protein 2, Xeplin, CMYA3, Cardiomyopathy-associated protein 3, Beta-xin

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  WB analysis of COLO 320 using 11896-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11896-1-AP (XIRP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Caco-2 using 11896-1-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 11896-1-AP (XIRP2 Antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 11896-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11896-1-AP (XIRP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human skeletal muscle using 11896-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 11896-1-AP (XIRP2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 11896-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 11896-1-AP (XIRP2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 11896-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11896-1-AP (XIRP2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 11896-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11896-1-AP (XIRP2 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of mouse heart using 11896-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using XIRP2 antibody (11896-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, Caco-2-Zellen, humanes Herzgewebe
• Erfolgreiche Detektion in IHC: humanes Skelettmuskelgewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mausherzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

11896-1-AP bindet in WB, IHC, IF-P, ELISA XIRP2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: XIRP2 fusion protein Ag2515
• Vollständiger Name: xin actin-binding repeat containing 2
• Berechnetes Molekulargewicht: 379 kDa
• Beobachtetes Molekulargewicht: 260-300 kDa
• GenBank-Zugangsnummer: BC022888
• Gene symbol: XIRP2
• Gene ID (NCBI): 129446
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB,IHC	Elife; Repair of noise-induced damage to stereocilia F-actin cores is facilitated by XIRP2 and its novel mechanosensor domain; Authors - Elizabeth L Wagner
mouse	WB,IF	Front Physiol; Transcriptome Analysis of Cardiac Hypertrophic Growth in MYBPC3-Null Mice Suggests Early Responders in Hypertrophic Remodeling.; Authors - Emily Farrell
human	WB,IF	Biology (Basel); Identification of Clinical Value and Biological Effects of XIRP2 Mutation in Hepatocellular Carcinoma; Authors - Dahuan Li ; KD Validated