XPA Polyklonaler Antikörper

XPA Polyklonal Antikörper für WB, IF/ICC, FC (Intra), IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, FC (Intra), IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16462-1-PBS

Synonyme

DNA repair protein complementing XP-A cells, Xeroderma pigmentosum group A-complementing protein, XP1, XPAC

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  WB analysis of L02 using 16462-1-AP (same clone as 16462-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16462-1-PBS in a different storage buffer formulation.

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  WB analysis of L02 using 16462-1-AP (same clone as 16462-1-PBS)

  L02 cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16462-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 16462-1-AP (same clone as 16462-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16462-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 16462-1-AP (same clone as 16462-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16462-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 16462-1-AP (same clone as 16462-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16462-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 16462-1-AP (same clone as 16462-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16462-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 16462-1-AP (same clone as 16462-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16462-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 16462-1-AP (same clone as 16462-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16462-1-PBS in a different storage buffer formulation.

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  IP experiment of L02 using 16462-1-AP (same clone as 16462-1-PBS)

  IP result of anti-XPA (IP:16462-1-AP, 4ug; Detection:16462-1-AP 1:300) with L02 cells lysate 3000 ug. This data was developed using the same antibody clone with 16462-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 16462-1-AP (same clone as 16462-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using XPA antibody (16462-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 16462-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 16462-1-AP (same clone as 16462-1-PBS)

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human XPA (16462-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 16462-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

16462-1-PBS bindet in WB, IF/ICC, FC (Intra), IP, Indirect ELISA XPA und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: XPA fusion protein Ag9536
• Vollständiger Name: xeroderma pigmentosum, complementation group A
• Berechnetes Molekulargewicht: 374aa,42 kDa; 273aa,31 kDa
• Beobachtetes Molekulargewicht: 40 kDa
• GenBank-Zugangsnummer: BC014965
• Gene symbol: XPA
• Gene ID (NCBI): 7507
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

XPA, also named as DNA repair protein complementing XP-A cells, is a 272 amino acid protein, which belongs to the XPA family. XPA is expressed in various cell lines and in skin fibroblasts. XPA is involved in DNA excision repair. Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. XPA is required for UV-induced CHEK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation. The calculated molecular weight of XPA is 31 kDa, but modified XPA protein is about 40 kDa (PMID: 17848622).