CRM1 Monoklonaler Antikörper

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• KD/KO Validated

CRM1 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(13)

CloneNo.

2D3B6

Kat-Nr. : 66763-1-Ig

Synonyme

XPO1, 2D3B6, Exp1, Exportin 1, Exportin-1

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  WB analysis using 66763-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66763-1-Ig (CRM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

• 

  WB analysis using 66763-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66763-1-Ig (CRM1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cervical cancer using 66763-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66763-1-Ig (CRM1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 66763-1-Ig

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66763-1-Ig (CRM1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen, PC-12-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Produktinformation

66763-1-Ig bindet in WB, IHC, IF, IP, ELISA CRM1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CRM1 fusion protein Ag27337
• Vollständiger Name: exportin 1 (CRM1 homolog, yeast)
• Berechnetes Molekulargewicht: 1071 aa, 123 kDa
• Beobachtetes Molekulargewicht: 100-110 kDa
• GenBank-Zugangsnummer: BC032847
• Gene symbol: CRM1
• Gene ID (NCBI): 7514
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CRM1 (chromosome region maintenance 1, also known as exportin-1 or XPO1), a member of the karyopherin β family of transport receptors, is an important nuclear export receptor recognizing proteins bearing a leucine-rich nuclear export signal (PMID: 9323133). CRM1 is the major receptor for the export of proteins out of the nucleus and is also required for transport of many RNAs (PMID: 17317185). In addition to nuclear export, CRM1 also plays a role in centrosome duplication and spindle assembly (PMID: 22773955). Elevated CRM1 expression has been reported in several cancers, which implicates CRM1 as a potential anti-cancer drug target (PMID: 22677130; 21683812 ).

Publikationen

Species	Application	Title
human	WB	Adv Sci (Weinh); Positive Feedback Regulation between KLF5 and XPO1 Promotes Cell Cycle Progression of Basal like Breast Cancer; Authors - Yu Tang ; KD Validated
rat,human	WB	Diabetologia; Saturated fatty acids entrap PDX1 in stress granules and impede islet beta cell function.; Authors - Mu Zhang
	WB	Sci Rep; Anti-tumor efficacy of Selinexor (KPT-330) in gastric cancer is dependent on nuclear accumulation of p53 tumor suppressor.; Authors - Vinod Vijay Subhash
mouse	WB	Exp Gerontol; Age-dependent changes in nuclear-cytoplasmic signaling in skeletal muscle.; Authors - Shama R Iyer
human	WB	Exp Cell Res; NEDD4 protects vascular endothelial cells against Angiotensin II-induced cell death via enhancement of XPO1-mediated nuclear export.; Authors - Jianning Xu
human	IHC	PeerJ; Hub gene associated with prognosis in bladder cancer is a novel therapeutic target; Authors - Dengpan Fang