XPR1 Rekombinanter Antikörper

XPR1 Rekombinant Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

241788A7

Kat-Nr. : 84902-1-RR

Synonyme

241788A7, Phosphate exporter SLC53A1, Protein SYG1 homolog, SLC53A1, Solute carrier family 53 member 1

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  WB analysis using 84902-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84902-1-RR (XPR1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 84902-1-RR

  IP result of anti-XPR1 (IP:84902-1-RR, 4ug; Detection:84902-1-RR 1:1000) with Jurkat cells lysate 1800 ug.

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  Affinity and Kinetic Characterization of 84902-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84902-1-RR against Human XPR1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, K-562-Zellen
• Erfolgreiche IP: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84902-1-RR bindet in WB, IP, ELISA XPR1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: XPR1 fusion protein Ag5373
• Vollständiger Name: xenotropic and polytropic retrovirus receptor
• Berechnetes Molekulargewicht: 696 aa, 82 kDa
• Beobachtetes Molekulargewicht: 74 kDa
• GenBank-Zugangsnummer: BC041142
• Gene symbol: XPR1
• Gene ID (NCBI): 9213
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

XPR1 is a receptor for xenotropic and polytropic murine leukemia retroviruses and a homolog of yeast Syg1 and plant Pi transporter PHO1 (PMID: 20633538). XPR1 has been identified as an atypical G-protein-coupled receptor. Xenotropic or polytropic retrovirus binding to XPR1 can disrupt the cAMP-mediated signaling function of Xpr1, leading to the apoptosis of infected cells (PMID: 22090134). A band of about 82-100 kDa is probably due to abnormal migration of the protein or post-translation modifications. In addition, a band of about 55-72 kDa may be isoforms and fragments.