XRCC2 Monoklonaler Antikörper

XRCC2 Monoklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite®555 Fluorescent Dye

CloneNo.

3D9A1

Kat-Nr. : CL555-66652

Synonyme

3D9A1, DNA repair protein XRCC2, X-ray repair cross-complementing protein 2

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  IF Staining of HepG2 using CL555-66652

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®555 XRCC2 antibody (CL555-66652, Clone: 3D9A1 ) at dilution of 1:200, CL488-phalloidin (green).

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  FC experiment of HepG2 using CL555-66652

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human XRCC2 (CL555-66652, Clone:3D9A1) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen
• Erfolgreiche Detektion in FC (Intra): HepG2-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL555-66652 bindet in IF/ICC, FC (Intra) XRCC2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: XRCC2 fusion protein Ag14421
• Vollständiger Name: X-ray repair complementing defective repair in Chinese hamster cells 2
• Berechnetes Molekulargewicht: 280 aa, 32 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: BC042137
• Gene symbol: XRCC2
• Gene ID (NCBI): 7516
• Konjugation: CoraLite®555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 557 nm / 570 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

XRCC2, also named as DNA repair protein XRCC2, is a 280 amino acid protein, which belongs to the RecA family. XRCC2 is involved in the homologous recombination repair (HRR) pathway of double-stranded DNA, thought to repair chromosomal fragmentation, translocations and deletions. XRCC2 is a part of the Rad21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway.