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XRCC4 Polyklonaler Antikörper

XRCC4 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte und mehr (1)

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(15)

Kat-Nr. : 15817-1-AP

Synonyme

X-ray repair cross-complementing protein 4, Protein XRCC4, C-terminus, hXRCC4, DNA repair protein XRCC4



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHepG2-Zellen, COLO 320-Zellen, HEK-293-Zellen
Erfolgreiche IPHepG2-Zellen
Erfolgreiche Detektion in IHChumanes Mammakarzinomgewebe, Ratten-Dünndarmgewebe, Rattenhodengewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

15817-1-AP bindet in WB, IHC, IF, IP, ELISA XRCC4 und zeigt Reaktivität mit human, Ratten

Getestete Reaktivität human, Ratte
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen XRCC4 fusion protein Ag8606
Vollständiger Name X-ray repair complementing defective repair in Chinese hamster cells 4
Berechnetes Molekulargewicht 310 aa, 35 kDa
Beobachtetes Molekulargewicht 55 kDa
GenBank-ZugangsnummerBC005259
Gene symbol XRCC4
Gene ID (NCBI) 7518
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

There are at least two pathways for eukaryotes to repair DNA double-strand breaks: homologous recombination and nonhomologous end joining(NHEJ). XRCC4 is one of the core machiney of NHEJ that required for double-strand break repair and V(D)J recombination. The DNA ligase IV(LIG4)-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for XRCC4 antibody 15817-1-APProtokoll herunterladen
IHC protocol for XRCC4 antibody 15817-1-APProtokoll herunterladenl
IP protocol for XRCC4 antibody 15817-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Nat Commun

Reciprocal regulation of RIG-I and XRCC4 connects DNA repair with RIG-I immune signaling.

Authors - Guijie Guo
  • KD Validated
humanWB

Cancer Res

Inhibition of ATM Induces Hypersensitivity to Proton Irradiation by Upregulating Toxic End Joining.

Authors - Qin Zhou

PLoS Biol

A long noncoding RNA sensitizes genotoxic treatment by attenuating ATM activation and homologous recombination repair in cancers.

Authors - Kunming Zhao
humanWB

Cell Death Differ

LRIK interacts with the Ku70-Ku80 heterodimer enhancing the efficiency of NHEJ repair.

Authors - Dan Wang
humanWB

Phytomedicine

Guiqi Baizhu decoction enhances radiosensitivity in non-small cell lung cancer by inhibiting the HIF-1α/DNA-PKcs axis-mediated DNA repair

Authors - Yangyang Li
humanWB

Toxicol Appl Pharmacol

Polychlorinated biphenyl quinone induces oxidative DNA damage and repair responses: The activations of NHEJ, BER and NER via ATM-p53 signaling axis.

Authors - Hui Dong

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Alexandra (Verified Customer) (07-02-2024)

For plasmid expression it works fine with 1000x dilution, but for the detection of endogenous XRCC4 I recommend the use of 500x dilution.

  • Applications: Western Blot, Immunofluorescence, Immunoprecipitation, Cell culture
  • Primary Antibody Dilution: 1000x
  • Cell Tissue Type: U2OS
XRCC4 Antibody Western Blot,Immunofluorescence,Immunoprecipitation,Cell culture validation (1000x dilution) in U2OS (Cat no:15817-1-AP)