XRCC4 Monoklonaler Antikörper

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XRCC4 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

3E5D2

Kat-Nr. : 66621-1-PBS

Synonyme

DNA repair protein XRCC4, XRCC4

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• 

  WB analysis using 66621-1-Ig (same clone as 66621-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66621-1-Ig (XRCC4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66621-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66621-1-Ig (same clone as 66621-1-PBS)

  WB result of XRCC4 antibody (66621-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-XRCC4 transfected HEK-293 cells. This data was developed using the same antibody clone with 66621-1-PBS in a different storage buffer formulation.

• 

  WB analysis of COLO 320 using 66621-1-Ig (same clone as 66621-1-PBS)

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 66621-1-Ig (XRCC4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66621-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 66621-1-Ig (same clone as 66621-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 66621-1-Ig (XRCC4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66621-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66621-1-Ig (same clone as 66621-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66621-1-Ig (XRCC4 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66621-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66621-1-Ig (same clone as 66621-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66621-1-Ig (XRCC4 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66621-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66621-1-Ig (same clone as 66621-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66621-1-Ig (XRCC4 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66621-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 66621-1-Ig (same clone as 66621-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66621-1-Ig (XRCC4 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66621-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66621-1-PBS bindet in WB, IHC, ELISA XRCC4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: XRCC4 fusion protein Ag8807
• Vollständiger Name: X-ray repair complementing defective repair in Chinese hamster cells 4
• Berechnetes Molekulargewicht: 310 aa, 35 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC005259
• Gene symbol: XRCC4
• Gene ID (NCBI): 7518
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.