XRCC5/Ku80 Polyklonaler Antikörper

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• KD/KO Validated

XRCC5/Ku80 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen(35)

Kat-Nr. : 16389-1-AP

Synonyme

KU80, XRCC5, double-strand-break rejoining, CTCBF, CTC85

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  WB analysis of HEK-293 using 16389-1-AP

  WB result of Ku80 antibody (16389-1-AP, 1:4000) with si-Control and si-Ku80 transfected HEK-293 cells.

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  WB analysis of HepG2 using 16389-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16389-1-AP (XRCC5/Ku80 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16389-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16389-1-AP (XRCC5/Ku80 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 16389-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16389-1-AP (XRCC5/Ku80 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 16389-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 16389-1-AP (XRCC5/Ku80 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16389-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16389-1-AP (XRCC5/Ku80 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 16389-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16389-1-AP (XRCC5/Ku80 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 16389-1-AP

  IP result of anti-XRCC5/Ku80 (IP:16389-1-AP, 4ug; Detection:16389-1-AP 1:1000) with HEK-293 cells lysate 1200ug.

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  IHC staining of human colon cancer using 16389-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16389-1-AP (XRCC5/Ku80 antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 16389-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16389-1-AP (XRCC5/Ku80 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 16389-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16389-1-AP (XRCC5/Ku80 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 16389-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16389-1-AP (XRCC5/Ku80 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 16389-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16389-1-AP (XRCC5/Ku80 antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 16389-1-AP

  Immunofluorescent analysis of HepG2 cells, using XRCC5 antibody 16389-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of HeLa using 16389-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using XRCC5/Ku80 antibody (16389-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, A431-Zellen, HEK-293-Zellen, HeLa-Zellen, humanes Lebergewebe, K-562-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:600-1:2400
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 30 publications below
• IHC: See 2 publications below
• IF: See 7 publications below
• IP: See 5 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below
• RIP: See 2 publications below

Produktinformation

16389-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA XRCC5/Ku80 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: XRCC5/Ku80 fusion protein Ag9454
• Vollständiger Name: X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)
• Berechnetes Molekulargewicht: 732 aa, 83 kDa
• Beobachtetes Molekulargewicht: 80-83 kDa
• GenBank-Zugangsnummer: BC019027
• Gene symbol: XRCC5
• Gene ID (NCBI): 7520
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

There are at least two pathways for eukaryotes to repair DNA double-strand breaks: homologous recombination and nonhomologous end joining(NHEJ). The core NHEJ machinery includes XRCC4, DNA ligase IV and the DNA-dependent protein kinase complex, which consists of the DNA end-binding XRCC5/XRCC6 heterodimer and the catalytic subunit PRKDC. The heterdimer of XRCC5/XRCC6 enhanced teh affinity of the catalytic subunit PRKDC to DNA by 100-fold. Once the XRCC5/6 dimer association with NAA15, it can bind to the osteocalcin promoter and activate osteocalcin expression. The XRCC5/6 dimer acts as a negative regulator of transcription when together with APEX1. Some publised papers indicated that the MW of XRCC5 is 86kDa, while more papers suggested that XRCC5 is a 80kDa protein, as it was firstly introducted in publication. Thus, Ku80 and Ku86 are the same protein.

Publikationen

Species	Application	Title
mouse,human	WB,IP	Immunity; Cytoplasmic DNA sensing by KU complex in aged CD4+ T cell potentiates T cell activation and aging-related autoimmune inflammation.; Authors - Yan Wang
human	IF	Oncogene; UBE2S, a novel substrate of Akt1, associates with Ku70 and regulates DNA repair and glioblastoma multiforme resistance to chemotherapy.; Authors - L Hu
human,mouse	WB,IHC,IP	Phytomedicine; Kaempferol inhibits non-homologous end joining repair via regulating Ku80 stability in glioma cancer; Authors - Meiyang Chen
human	WB	J Mater Chem B; Hollow PtCo alloy nanospheres as a high-Z and oxygen generating nanozyme for radiotherapy enhancement in non-small cell lung cancer; Authors - Shuying Li
human	IF	Mol Med; Nuclear VPS35 attenuates NHEJ repair by sequestering Ku protein; Authors - Luping Zhang
human	WB	FEBS J; Radiation resistance of cancer cells caused by mitochondrial dysfunction depends on SIRT3-mediated mitophagy; Authors - Yan Wei