XRCC5/Ku80 Rekombinanter Antikörper

XRCC5/Ku80 Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240301G6

Kat-Nr. : 80325-6-PBS

Synonyme

KU80, XRCC5, 240301G6, ATP-dependent DNA helicase 2 subunit 2, ATP-dependent DNA helicase II 80 kDa subunit

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  WB analysis using 80325-6-RR (same clone as 80325-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80325-6-RR (XRCC5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80325-6-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 80325-6-RR (same clone as 80325-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80325-6-RR (XRCC5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80325-6-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 80325-6-RR (same clone as 80325-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80325-6-RR (XRCC5 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80325-6-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 80325-6-RR (same clone as 80325-6-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using XRCC5 antibody (80325-6-RR, Clone: 240301G6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 80325-6-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 80325-6-RR (same clone as 80325-6-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug XRCC5 Recombinant antibody (80325-6-RR, Clone:240301G6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 80325-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 80325-6-RR

  Biolayer interferometry (BLl) kinetic assays of 80325-6-RR against Human XRCC5 were performed. The affinity constant is 4.59 nM.

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Geprüfte Anwendungen

Produktinformation

80325-6-PBS bindet in WB, IHC, IF/ICC, FC (Intra), ELISA XRCC5/Ku80 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: XRCC5/Ku80 fusion protein Ag9454
• Vollständiger Name: X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)
• Berechnetes Molekulargewicht: 732 aa, 83 kDa
• Beobachtetes Molekulargewicht: 80 kDa
• GenBank-Zugangsnummer: BC019027
• Gene symbol: XRCC5
• Gene ID (NCBI): 7520
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

There are at least two pathways for eukaryotes to repair DNA double-strand breaks: homologous recombination and nonhomologous end joining(NHEJ). The core NHEJ machinery includes XRCC4, DNA ligase IV and the DNA-dependent protein kinase complex, which consists of the DNA end-binding XRCC5/XRCC6 heterodimer and the catalytic subunit PRKDC. The heterdimer of XRCC5/XRCC6 enhanced teh affinity of the catalytic subunit PRKDC to DNA by 100-fold. Once the XRCC5/6 dimer association with NAA15, it can bind to the osteocalcin promoter and activate osteocalcin expression. The XRCC5/6 dimer acts as a negative regulator of transcription when together with APEX1. Some publised papers indicated that the MW of XRCC5 is 86kDa, while more papers suggested that XRCC5 is a 80kDa protein, as it was firstly introducted in publication.