XRCC5/Ku80 Monoklonaler Antikörper

XRCC5/Ku80 Monoklonal Antikörper für IF/ICC, FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

2G5E7

Kat-Nr. : CL594-66546

Synonyme

KU80, XRCC5, 2G5E7, ATP-dependent DNA helicase 2 subunit 2, ATP-dependent DNA helicase II 80 kDa subunit

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  IF Staining of HeLa using CL594-66546

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594-conjugated XRCC5 antibody (CL594-66546, Clone: 2G5E7 ) at dilution of 1:100.

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  IF Staining of HeLa using CL594-66546

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 XRCC5 antibody (CL594-66546, Clone: 2G5E7 ) at dilution of 1:200, CL488-phalloidin (green).

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  IF Staining of HeLa using CL594-66546

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CL594-66546 (XRCC5 antibody) at dilution of 1:100.

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  FC experiment of HeLa using CL594-66546

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite®594 Anti-Human XRCC5 (CL594-66546, Clone:2G5E7) (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL594-66546 bindet in IF/ICC, FC (Intra) XRCC5/Ku80 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: XRCC5/Ku80 fusion protein Ag9512
• Vollständiger Name: X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining)
• Berechnetes Molekulargewicht: 732 aa, 83 kDa
• Beobachtetes Molekulargewicht: 80-83 kDa
• GenBank-Zugangsnummer: BC019027
• Gene symbol: XRCC5
• Gene ID (NCBI): 7520
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

There are at least two pathways for eukaryotes to repair DNA double-strand breaks: homologous recombination and nonhomologous end joining(NHEJ). The core NHEJ machinery includes XRCC4, DNA ligase IV and the DNA-dependent protein kinase complex, which consists of the DNA end-binding XRCC5/XRCC6 heterodimer and the catalytic subunit PRKDC. The heterdimer of XRCC5/XRCC6 enhanced teh affinity of the catalytic subunit PRKDC to DNA by 100-fold. Once the XRCC5/6 dimer association with NAA15, it can bind to the osteocalcin promoter and activate osteocalcin expression. The XRCC5/6 dimer acts as a negative regulator of transcription when together with APEX1. Some publised papers indicated that the MW of XRCC5 is 86kDa, while more papers suggested that XRCC5 is a 80kDa protein, as it was firstly introducted in publication. Thus, Ku80 and Ku86 are the same protein.