XRCC5 Monoklonaler Antikörper
XRCC5 Monoklonal Antikörper für FC (Intra)
Wirt / Isotyp
Maus / IgG1
Getestete Reaktivität
human, Maus, Ratte
Anwendung
FC (Intra)
Konjugation
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
2G5E7
Kat-Nr. : CL647-66546
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in FC (Intra) | HeLa-Zellen | 
| Erfolgreiche Detektion in FC | HeLa-Zellen | 
Empfohlene Verdünnung
| Anwendung | Verdünnung | 
|---|---|
| Durchflusszytometrie (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension | 
| Durchflusszytometrie (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Produktinformation
CL647-66546 bindet in FC (Intra) XRCC5 und zeigt Reaktivität mit human, Maus, Ratten
| Getestete Reaktivität | human, Maus, Ratte | 
| Wirt / Isotyp | Maus / IgG1 | 
| Klonalität | Monoklonal | 
| Typ | Antikörper | 
| Immunogen | XRCC5 fusion protein Ag9512 | 
| Vollständiger Name | X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining) | 
| Berechnetes Molekulargewicht | 732 aa, 83 kDa | 
| Beobachtetes Molekulargewicht | 80-83 kDa | 
| GenBank-Zugangsnummer | BC019027 | 
| Gene symbol | XRCC5 | 
| Gene ID (NCBI) | 7520 | 
| Konjugation | CoraLite® Plus 647 Fluorescent Dye | 
| Excitation/Emission maxima wavelengths | 654 nm / 674 nm | 
| Form | Liquid | 
| Reinigungsmethode | Protein-G-Reinigung | 
| Lagerungspuffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA | 
| Lagerungsbedingungen | Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. | 
Hintergrundinformationen
There are at least two pathways for eukaryotes to repair DNA double-strand breaks: homologous recombination and nonhomologous end joining(NHEJ). The core NHEJ machinery includes XRCC4, DNA ligase IV and the DNA-dependent protein kinase complex, which consists of the DNA end-binding XRCC5/XRCC6 heterodimer and the catalytic subunit PRKDC. The heterdimer of XRCC5/XRCC6 enhanced teh affinity of the catalytic subunit PRKDC to DNA by 100-fold. Once the XRCC5/6 dimer association with NAA15, it can bind to the osteocalcin promoter and activate osteocalcin expression. The XRCC5/6 dimer acts as a negative regulator of transcription when together with APEX1. Some publised papers indicated that the MW of XRCC5 is 86kDa, while more papers suggested that XRCC5 is a 80kDa protein, as it was firstly introducted in publication. Thus, Ku80 and Ku86 are the same protein.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| FC protocol for CL Plus 647 XRCC5 antibody CL647-66546 | Download protocol | 
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen | 
