XRN2 Rekombinanter Antikörper

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• KD/KO Validated

XRN2 Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240065G4

Kat-Nr. : 83378-1-RR

Synonyme

240065G4, 5 3 exoribonuclease 2, 5' 3' exoribonuclease 2, 5'-3' exoribonuclease 2, DHM1 like protein

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  WB analysis of MCF-7 using 83378-1-RR

  WB result of XRN2 antibody (83378-1-RR; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-XRN2 transfected MCF-7 cells.

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  WB analysis using 83378-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83378-1-RR (XRN2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 83378-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug XRN2 Recombinant antibody (83378-1-RR, Clone:240065G4) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 83378-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83378-1-RR against Human XRN2 were performed. The affinity constant is 1.49 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, HeLa-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83378-1-RR bindet in WB, FC (Intra), ELISA XRN2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: XRN2 fusion protein Ag30781
• Vollständiger Name: 5'-3' exoribonuclease 2
• Berechnetes Molekulargewicht: 104 kDa
• Beobachtetes Molekulargewicht: 109 kDa
• GenBank-Zugangsnummer: BC006417
• Gene symbol: XRN2
• Gene ID (NCBI): 22803
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

XRN2 is one exonuclease that degrades the Pol II associated product of poly(A) site cleavage, which is crucial for Pol II termination. During transcription termination, XRN2 cleavages at the polyadenylation site liberates a 5' fragment which is subsequently processed to form the mature mRNA and a 3' fragment which remains attached to the elongating polymerase. The processive degradation of this 3' fragment by this protein may promote termination of transcription.