YAP1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

YAP1 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13584-1-PBS

Synonyme

65 kDa Yes associated protein, Protein yorkie homolog, Transcriptional coactivator YAP1, YAP, yes associated protein 1

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  WB analysis using 13584-1-AP (same clone as 13584-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13584-1-AP (YAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13584-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 13584-1-AP (same clone as 13584-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13584-1-AP (YAP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13584-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 13584-1-AP (same clone as 13584-1-PBS)

  WB result of YAP1 antibody (13584-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YAP1 transfected HepG2 cells. This data was developed using the same antibody clone with 13584-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 13584-1-AP (same clone as 13584-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13584-1-AP (YAP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13584-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 13584-1-AP (same clone as 13584-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13584-1-AP (YAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13584-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 13584-1-AP (same clone as 13584-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13584-1-AP (YAP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13584-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 13584-1-AP (same clone as 13584-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 13584-1-AP (YAP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13584-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 13584-1-AP (same clone as 13584-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 13584-1-AP (YAP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13584-1-PBS in a different storage buffer formulation.

• 

  IP experiment of NIH/3T3 using 13584-1-AP (same clone as 13584-1-PBS)

  IP result of anti-YAP1 (IP:13584-1-AP, 3ug; Detection:13584-1-AP 1:700) with NIH/3T3 cells lysate 1200ug. This data was developed using the same antibody clone with 13584-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 13584-1-AP (same clone as 13584-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13584-1-AP (YAP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13584-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 13584-1-AP (same clone as 13584-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13584-1-AP (YAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13584-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 13584-1-AP (same clone as 13584-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13584-1-AP (YAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13584-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 13584-1-AP (same clone as 13584-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13584-1-AP (YAP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13584-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 13584-1-AP (same clone as 13584-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13584-1-AP (YAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13584-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 13584-1-AP (same clone as 13584-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13584-1-AP (YAP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13584-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 13584-1-AP (same clone as 13584-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13584-1-AP (YAP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13584-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 13584-1-AP (same clone as 13584-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13584-1-AP (YAP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13584-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 13584-1-AP (same clone as 13584-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13584-1-AP (YAP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13584-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 13584-1-AP (same clone as 13584-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 13584-1-AP (YAP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13584-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 13584-1-AP (same clone as 13584-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13584-1-AP (YAP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13584-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human lung cancer using 13584-1-AP (same clone as 13584-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using YAP1 antibody (13584-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13584-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human lung cancer using 13584-1-AP (same clone as 13584-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using YAP1 antibody (13584-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13584-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 13584-1-AP (same clone as 13584-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 13584-1-AP (YAP1 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 13584-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 13584-1-AP (same clone as 13584-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using YAP1 antibody (13584-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13584-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13584-1-PBS bindet in WB, IHC, IF/ICC, IF-P, IP, ELISA YAP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: YAP1 fusion protein Ag4510
• Vollständiger Name: Yes-associated protein 1, 65kDa
• Berechnetes Molekulargewicht: 504 aa, 54 kDa
• Beobachtetes Molekulargewicht: 65-75 kDa
• GenBank-Zugangsnummer: BC038235
• Gene symbol: YAP1
• Gene ID (NCBI): 10413
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Yes-associated protein 1 (YAP1) is a transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3).Increased expression seen in some liver and prostate cancers (PMID: 31613226, 32488048, 33520338). Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level).It is actived by phosphorylation and degradated by ubiquitination (PMID: 20048001).This antibody is a rabbit polyclonal antibody. The calcualted molecular weight of YAP1 is 54 kDa, but routinely observed at 65-75 kDa by Western Blot (PMID: 28230103, 33264286, 36255405).