YAP1 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

YAP1 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(82)

CloneNo.

3A7A9

Rezensionen (2)

Kat-Nr. : 66900-1-Ig

Synonyme

yes associated protein 1, YAP, Transcriptional coactivator YAP1, Protein yorkie homolog, 65 kDa Yes associated protein

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  WB analysis using 66900-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66900-1-Ig (YAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

• 

  WB analysis using 66900-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66900-1-Ig (YAP1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66900-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66900-1-Ig (YAP1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 66900-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66900-1-Ig (YAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 66900-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66900-1-Ig (YAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66900-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66900-1-Ig (YAP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 66900-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66900-1-Ig (YAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 66900-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66900-1-Ig (YAP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66900-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66900-1-Ig (YAP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66900-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66900-1-Ig (YAP1 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66900-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66900-1-Ig (YAP1 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 66900-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66900-1-Ig (YAP1 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66900-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66900-1-Ig (YAP1 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 66900-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66900-1-Ig (YAP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 66900-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66900-1-Ig (YAP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 66900-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using YAP1 antibody (66900-1-Ig, Clone: 3A7A9 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NCI-H1299-Zellen, 4T1-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, HUVEC-Zellen, LNCaP-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, NIH-3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Leberkarzinomgewebe, humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 12 publications below
• WB: See 65 publications below
• IHC: See 20 publications below
• IF: See 46 publications below
• IP: See 4 publications below
• CoIP: See 7 publications below

Produktinformation

66900-1-Ig bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA YAP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: YAP1 fusion protein Ag28194
• Vollständiger Name: Yes-associated protein 1, 65kDa
• Berechnetes Molekulargewicht: 504 aa, 54 kDa
• Beobachtetes Molekulargewicht: 65-75 kDa
• GenBank-Zugangsnummer: BC038235
• Gene symbol: YAP1
• Gene ID (NCBI): 10413
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Yes-associated protein 1 (YAP1) is a transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3).Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level). Phosphorylation of S381 primes YAP phosphorylation by CK1δ/ɛ, resulting in activation of a phosphodegron, thus generates a binding surface that interacts with a ubiquitin ligase, and leads to degradation by ubiquitination. (PMID: 20048001). The calcualted molecular weight of YAP1 is 54 kDa, but routinely observed at 65-75 kDa by Western Blot (PMID: 28230103, 33264286, 36255405).

Publikationen

Species	Application	Title
human	WB	Mol Cancer; Co-transcriptional R-loops-mediated epigenetic regulation drives growth retardation and docetaxel chemosensitivity enhancement in advanced prostate cancer; Authors - Yufan Ying
human	IF	Nat Commun; Endothelial discoidin domain receptor 1 senses flow to modulate YAP activation; Authors - Jiayu Liu
human	WB	Nat Commun; Fusobacterium nucleatum reduces METTL3-mediated m6A modification and contributes to colorectal cancer metastasis.; Authors - Shujie Chen ; KD Validated
human	WB,IHC	Cancer Lett; A novel antagonist of the CCL5/CCR5 axis suppresses the tumor growth and metastasis of triple-negative breast cancer by CCR5-YAP1 regulation; Authors - Ling Chen ; KD Validated
human,mouse	WB,IHC,IF,CoIP	Acta Pharmacol Sin; Adaptor protein CEMIP reduces the chemosensitivity of small cell lung cancer via activation of an SRC-YAP oncogenic module; Authors - Xiao-Ju Shen
human	WB,IF	Oncogene; The keratin 17/YAP/IL6 axis contributes to E-cadherin loss and aggressiveness of diffuse gastric cancer; Authors - Mengjie Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Udesh (Verified Customer) (11-26-2023)

Worked well at 1:1000 for WB

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Glioblastoma

FH

Joshua (Verified Customer) (01-29-2019)

Coverslips were fixed with 4%PFA+0.25% Triton-X-100. Primary staining was 1:500, secondary was 1:750 with rockland 488 anti-mouse overnight at 4C.Left panel: Control PANC1 cells.Right panel: PANC1 cells with YAP silencing

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: PANC1

YAP1 Antibody Immunofluorescence, validation (1:500 dilution) in PANC1 (Cat no:66900-1-Ig)