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  • KD/KO Validated

YAP1 Monoklonaler Antikörper

YAP1 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(82)

CloneNo.

3A7A9

Kat-Nr. : 66900-1-Ig

Synonyme

yes associated protein 1, YAP, Transcriptional coactivator YAP1, Protein yorkie homolog, 65 kDa Yes associated protein



Geprüfte Anwendungen

Erfolgreiche Detektion in WBNCI-H1299-Zellen, 4T1-Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, HUVEC-Zellen, LNCaP-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, NIH-3T3-Zellen
Erfolgreiche Detektion in IHChumanes Lungenkarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Leberkarzinomgewebe, humanes Ovarialkarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunhistochemie (IHC)IHC : 1:1000-1:4000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

66900-1-Ig bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA YAP1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen YAP1 fusion protein Ag28194
Vollständiger Name Yes-associated protein 1, 65kDa
Berechnetes Molekulargewicht 504 aa, 54 kDa
Beobachtetes Molekulargewicht65-75 kDa
GenBank-ZugangsnummerBC038235
Gene symbol YAP1
Gene ID (NCBI) 10413
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Yes-associated protein 1 (YAP1) is a transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3).Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level). Phosphorylation of S381 primes YAP phosphorylation by CK1δ/ɛ, resulting in activation of a phosphodegron, thus generates a binding surface that interacts with a ubiquitin ligase, and leads to degradation by ubiquitination. (PMID: 20048001). The calcualted molecular weight of YAP1 is 54 kDa, but routinely observed at 65-75 kDa by Western Blot (PMID: 28230103, 33264286, 36255405).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for YAP1 antibody 66900-1-IgProtokoll herunterladen
IHC protocol for YAP1 antibody 66900-1-IgProtokoll herunterladenl
IF protocol for YAP1 antibody 66900-1-IgProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Mol Cancer

Co-transcriptional R-loops-mediated epigenetic regulation drives growth retardation and docetaxel chemosensitivity enhancement in advanced prostate cancer

Authors - Yufan Ying
humanIF

Nat Commun

Endothelial discoidin domain receptor 1 senses flow to modulate YAP activation

Authors - Jiayu Liu
humanWB

Nat Commun

Fusobacterium nucleatum reduces METTL3-mediated m6A modification and contributes to colorectal cancer metastasis.

Authors - Shujie Chen
  • KD Validated
humanWB,IHC

Cancer Lett

A novel antagonist of the CCL5/CCR5 axis suppresses the tumor growth and metastasis of triple-negative breast cancer by CCR5-YAP1 regulation

Authors - Ling Chen
  • KD Validated
human,mouseWB,IHC,IF,CoIP

Acta Pharmacol Sin

Adaptor protein CEMIP reduces the chemosensitivity of small cell lung cancer via activation of an SRC-YAP oncogenic module

Authors - Xiao-Ju Shen
humanWB,IF

Oncogene

The keratin 17/YAP/IL6 axis contributes to E-cadherin loss and aggressiveness of diffuse gastric cancer

Authors - Mengjie Li

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Udesh (Verified Customer) (11-26-2023)

Worked well at 1:1000 for WB

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Glioblastoma
FH

Joshua (Verified Customer) (01-29-2019)

Coverslips were fixed with 4%PFA+0.25% Triton-X-100. Primary staining was 1:500, secondary was 1:750 with rockland 488 anti-mouse overnight at 4C.Left panel: Control PANC1 cells.Right panel: PANC1 cells with YAP silencing

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: PANC1
YAP1 Antibody Immunofluorescence, validation (1:500 dilution) in PANC1 (Cat no:66900-1-Ig)