YAP1 Monoklonaler Antikörper

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• KD/KO Validated

YAP1 Monoklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

3A7A9

Kat-Nr. : 66900-1-PBS

Synonyme

yes associated protein 1, YAP, Transcriptional coactivator YAP1, Protein yorkie homolog, 65 kDa Yes associated protein

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  WB analysis using 66900-1-Ig (same clone as 66900-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66900-1-Ig (YAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66900-1-PBS in a different storage buffer formulation.

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  WB analysis using 66900-1-Ig (same clone as 66900-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66900-1-Ig (YAP1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66900-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66900-1-Ig (same clone as 66900-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66900-1-Ig (YAP1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66900-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66900-1-Ig (same clone as 66900-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66900-1-Ig (YAP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66900-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66900-1-Ig (same clone as 66900-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66900-1-Ig (YAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66900-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 66900-1-Ig (same clone as 66900-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66900-1-Ig (YAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66900-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 66900-1-Ig (same clone as 66900-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66900-1-Ig (YAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66900-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66900-1-Ig (same clone as 66900-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66900-1-Ig (YAP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66900-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66900-1-Ig (same clone as 66900-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66900-1-Ig (YAP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66900-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66900-1-Ig (same clone as 66900-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66900-1-Ig (YAP1 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66900-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66900-1-Ig (same clone as 66900-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66900-1-Ig (YAP1 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66900-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66900-1-Ig (same clone as 66900-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66900-1-Ig (YAP1 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66900-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66900-1-Ig (same clone as 66900-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66900-1-Ig (YAP1 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66900-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 66900-1-Ig (same clone as 66900-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66900-1-Ig (YAP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66900-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 66900-1-Ig (same clone as 66900-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66900-1-Ig (YAP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66900-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66900-1-Ig (same clone as 66900-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using YAP1 antibody (66900-1-Ig, Clone: 3A7A9 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66900-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66900-1-PBS bindet in WB, IHC, IF/ICC, ELISA YAP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: YAP1 fusion protein Ag28194
• Vollständiger Name: Yes-associated protein 1, 65kDa
• Berechnetes Molekulargewicht: 504 aa, 54 kDa
• Beobachtetes Molekulargewicht: 65-75 kDa
• GenBank-Zugangsnummer: BC038235
• Gene symbol: YAP1
• Gene ID (NCBI): 10413
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Yes-associated protein 1 (YAP1) is a transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3).Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level). Phosphorylation of S381 primes YAP phosphorylation by CK1δ/ɛ, resulting in activation of a phosphodegron, thus generates a binding surface that interacts with a ubiquitin ligase, and leads to degradation by ubiquitination. (PMID: 20048001). The calcualted molecular weight of YAP1 is 54 kDa, but routinely observed at 65-75 kDa by Western Blot (PMID: 28230103, 33264286, 36255405).