YARS Rekombinanter Antikörper

YARS Rekombinant Antikörper für WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human

Anwendung

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242400E9

Kat-Nr. : 84859-3-PBS

Synonyme

YARS1, 242400E9, CMTDIC, TYRRS, YRS

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  WB analysis using 84859-3-RR (same clone as 84859-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84859-3-RR (YARS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84859-3-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 84859-3-RR (same clone as 84859-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84859-3-RR (YARS antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84859-3-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 84859-3-RR (same clone as 84859-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84859-3-RR (YARS antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84859-3-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 84859-3-RR (same clone as 84859-3-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using YARS antibody (84859-3-RR, Clone: 242400E9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84859-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01634-2

  Cytometric bead array standard curve of MP01634-2, YARS Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84859-3-PBS. Detection antibody: 84859-2-PBS. Standard: Ag35148. Range: 0.156-20 ng/mL.

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  Affinity and Kinetic Characterization of 84859-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84859-3-RR against Human YARS were performed. The affinity constant is 0.414 nM.

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Geprüfte Anwendungen

Produktinformation

84859-3-PBS bindet in WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA YARS und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: YARS fusion protein Ag35148
• Vollständiger Name: tyrosyl-tRNA synthetase
• Berechnetes Molekulargewicht: 59 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC016689
• Gene symbol: YARS
• Gene ID (NCBI): 8565
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Tyrosyl-tRNA synthetase (YARS) is responsible for tyrosyl-tRNA aminoacylation. The functional enzyme exists as a homodimer, has potent leukocyte and monocyte chemotaxis activity, and stimulates production of myeloperoxidase, tumor necrosis factor-α, and tissue factor. A shift in the monomer:dimer equilibrium could result in dysregulation of the cytokines function. (PMID: 33490854)