YES1 Monoklonaler Antikörper

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• KD/KO Validated

YES1 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1A12E7

Kat-Nr. : 67196-1-PBS

Synonyme

c yes, HsT441, P61 YES, Proto oncogene c Yes, Yes, YES1

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  WB analysis using 67196-1-Ig (same clone as 67196-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67196-1-Ig (YES1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67196-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 67196-1-Ig (same clone as 67196-1-PBS)

  WB result of YES1 antibody (67196-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-YES1 transfected HepG2 cells. This data was developed using the same antibody clone with 67196-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 67196-1-Ig (same clone as 67196-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67196-1-Ig (YES1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67196-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 67196-1-Ig (same clone as 67196-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67196-1-Ig (YES1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67196-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67196-1-PBS bindet in WB, IHC, Indirect ELISA YES1 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: YES1 fusion protein Ag5016
• Vollständiger Name: v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1
• Berechnetes Molekulargewicht: 61 kDa
• Beobachtetes Molekulargewicht: 62 kDa
• GenBank-Zugangsnummer: BC048960
• Gene symbol: YES1
• Gene ID (NCBI): 7525
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

YES1, also named as p61-Yes and YES, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It promotes infectivity of Neisseria gonorrhoeae in epithelial cells by phosphorylating MCP/CD46. YES1 catalyzes the reaction: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.