CD20 Monoklonaler Antikörper

CD20 Monoklonal Antikörper für IF/ICC, IF-P

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

IF/ICC, IF-P

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4A7G3

Kat-Nr. : CL488-60271

Synonyme

B lymphocyte antigen CD20, B1, Bp35, CD20, LEU 16, MS4A1, MS4A1,CD20, MS4A2, S7

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  IF Staining of human tonsillitis using CL488-60271

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL488-60271 (CD20 antibody) at dilution of 1:100.

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  IF Staining of human tonsillitis using CL488-60271

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL488-60271 (CD20 antibody) at dilution of 1:100.

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  IF Staining of human lung cancer using CL488-60271

  Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite® Plus 488 CD20 antibody (CL488-60271, Clone: 4A7G3 ) at dilution of 1:100, CoraLite®555 pan-keratin antibody (CL555-26411, Gradient).

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  IF Staining of Raji using CL488-60271

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using CoraLite® Plus 488 CD20 antibody (CL488-60271, Clone: 4A7G3 ) at dilution of 1:200.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF-P: humanes Tonsillitisgewebe, humanes Lungenkarzinomgewebe, Raji-Zellen
• Erfolgreiche Detektion in IF/ICC: humanes Tonsillitisgewebe, humanes Lungenkarzinomgewebe, Raji-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL488-60271 bindet in IF/ICC, IF-P CD20 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: membrane-spanning 4-domains, subfamily A, member 1
• Berechnetes Molekulargewicht: 297 aa, 33 kDa
• GenBank-Zugangsnummer: BC002807
• Gene symbol: CD20
• Gene ID (NCBI): 931
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD20 is a 33-37 kDa transmembrane phosphoprotein. CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells. CD20 functions as calcium-permeable cation channel. It is involved in the regulation of B-cell activation and proliferation. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B-cells. This antibody is conjugated with CL488(Ex/Em 488 nm/515 nm).