YIPF2 Polyklonaler Antikörper

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• KD/KO Validated

YIPF2 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20441-1-AP

Synonyme

FinGER2, Protein YIPF2, Yip1 domain family, member 2, YIP1 family member 2

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  WB analysis of HepG2 using 20441-1-AP

  WB result of YIPF2 antibody (20441-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YIPF2 transfected HepG2 cells.

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  WB analysis using 20441-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20441-1-AP (YIPF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 20441-1-AP

  IP result of anti-YIPF2 (IP:20441-1-AP, 4ug; Detection:20441-1-AP 1:500) with HepG2 cells lysate 1470 ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen
• Erfolgreiche IP: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

20441-1-AP bindet in WB, IP, ELISA YIPF2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: YIPF2 fusion protein Ag14238
• Vollständiger Name: Yip1 domain family, member 2
• Berechnetes Molekulargewicht: 316 aa, 35 kDa
• Beobachtetes Molekulargewicht: 35~40 kDa
• GenBank-Zugangsnummer: BC000056
• Gene symbol: YIPF2
• Gene ID (NCBI): 78992
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

YIPF2 is a member of YIP family whose name refers to the Ypt (yeast RAB GTPase)-interacting protein predicted to have five transmembrane segments with an N-terminal exposed to the cytoplasm and a short C-terminal exposed to the lumen of the secretory pathway. YIPF2 has been reported to mainly locate in the trans-Golgi network (TGN) co-localized with virous RAB proteins, suggesting that it is potentially involved in vesicle transport (PMID: 31189879). YIPF2 can serve as the GDF (GDI-displacement factor) of RAB5/RAB22A and intracellular binder of CD147 in HCC cells (PMID: 311898799).