YIPF4 Polyklonaler Antikörper

YIPF4 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15473-1-PBS

Synonyme

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• 

  WB analysis of HeLa using 15473-1-PBS

  HeLa cells were subjected to SDS PAGE followed by western blot with 15473-1-AP (YIPF4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15473-1-AP (same clone as 15473-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15473-1-AP (YIPF4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15473-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 15473-1-PBS

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15473-1-AP (YIPF4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human endometrial cancer using 15473-1-AP (same clone as 15473-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 15473-1-AP (YIPF4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15473-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human endometrial cancer using 15473-1-AP (same clone as 15473-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 15473-1-AP (YIPF4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15473-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 15473-1-AP (same clone as 15473-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using YIPF4 antibody (15473-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 15473-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 15473-1-AP (same clone as 15473-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15473-1-AP (YIPF4 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Red: CL594-phalloidin staining of F-actin. This data was developed using the same antibody clone with 15473-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15473-1-PBS bindet in WB, IHC, IF/ICC, ELISA YIPF4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: YIPF4 fusion protein Ag7777
• Vollständiger Name: Yip1 domain family, member 4
• Berechnetes Molekulargewicht: 27 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC004875
• Gene symbol: YIPF4
• Gene ID (NCBI): 84272
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.