YME1L1 Polyklonaler Antikörper

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• KD/KO Validated

YME1L1 Polyklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11510-1-PBS

Synonyme

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  WB analysis using 11510-1-AP (same clone as 11510-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11510-1-AP (YME1L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11510-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 11510-1-AP (same clone as 11510-1-PBS)

  WB result of YME1L1 antibody (11510-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YME1L1 transfected HeLa cells. This data was developed using the same antibody clone with 11510-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 11510-1-AP (same clone as 11510-1-PBS)

  IP result of anti-YME1L1 (IP:11510-1-AP, 4ug; Detection:11510-1-AP 1:500) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 11510-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 11510-1-AP (same clone as 11510-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using YME1L1 antibody (11510-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11510-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

11510-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA YME1L1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: YME1L1 fusion protein Ag2069
• Vollständiger Name: YME1-like 1 (S. cerevisiae)
• Berechnetes Molekulargewicht: 716 aa, 80 kDa
• Beobachtetes Molekulargewicht: 63 kDa
• GenBank-Zugangsnummer: BC023507
• Gene symbol: YME1L1
• Gene ID (NCBI): 10730
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

YME1L1(ATP-dependent zinc metalloprotease) is also named as FTSH1, YME1L,Meg-4,PAMP. YME1L1 plays a phylogenetically conserved role in mitochondrial protein metabolism.It also ensures cell proliferation, maintains normal cristae morphology and complex I respiration activity, promotes antiapoptotic activity and protects mitochondria from the accumulation of oxidatively damaged membrane proteins. YME1L1 can prevent mitochondrial DNA inserting into nuclear. It has 3 isoforms produced by alternative splicing with the molecular weight of 86 kDa, 80 kDa and 76 kDa. This protein can migrate with a molecular weight of about 55-63 kDa, which is the size of the mature YME1L1 protein(PMID:22252130; 22354088).