YME1L1 Monoklonaler Antikörper

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• KD/KO Validated

YME1L1 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2B7E4

Kat-Nr. : 66551-1-PBS

Synonyme

2B7E4, ATP-dependent metalloprotease FtsH1, ATP-dependent zinc metalloprotease YME1L1, EC:3.4.24.-, FTSH

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  WB analysis of HeLa using 66551-1-Ig (same clone as 66551-1-PBS)

  WB result of YME1L1 antibody (66551-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YME1L1 transfected HeLa cells. This data was developed using the same antibody clone with 66551-1-PBS in a different storage buffer formulation.

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  WB analysis using 66551-1-Ig (same clone as 66551-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66551-1-Ig (YME1L1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66551-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 66551-1-Ig (same clone as 66551-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66551-1-Ig (YME1L1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66551-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66551-1-Ig (same clone as 66551-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66551-1-Ig (YME1L1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66551-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66551-1-Ig (same clone as 66551-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66551-1-Ig (YME1L1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66551-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66551-1-PBS bindet in WB, IHC, Indirect ELISA YME1L1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: YME1L1 fusion protein Ag2069
• Vollständiger Name: YME1-like 1 (S. cerevisiae)
• Berechnetes Molekulargewicht: 716 aa, 80 kDa
• Beobachtetes Molekulargewicht: 63 kDa
• GenBank-Zugangsnummer: BC023507
• Gene symbol: YME1L1
• Gene ID (NCBI): 10730
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

YME1L1(ATP-dependent zinc metalloprotease) is also named as FTSH1, YME1L,Meg-4,PAMP. YME1L1 plays a phylogenetically conserved role in mitochondrial protein metabolism.It also ensures cell proliferation, maintains normal cristae morphology and complex I respiration activity, promotes antiapoptotic activity and protects mitochondria from the accumulation of oxidatively damaged membrane proteins. YME1L1 can prevent mitochondrial DNA inserting into nuclear. It has 3 isoforms produced by alternative splicing with the molecular weight of 86 kDa, 80 kDa and 76 kDa. This protein can migrate with a molecular weight of about 55-63 kDa, which is the size of the mature YME1L1 protein(PMID:22252130; 22354088).