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YOD1 Rekombinanter Antikörper

YOD1 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242498G6

Kat-Nr. : 84933-5-PBS

Synonyme

242498G6, DUBA 8, DUBA8, DUBA-8, EC:3.4.19.12

Filter:
  • Western Blot (WB) analysis of various lysates using YOD1 Recombinant antibody (84933-5-RR)

    WB analysis using 84933-5-RR (same clone as 84933-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84933-5-RR (YOD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84933-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of K-562 cells using YOD1 Recombinant antibody (84933-5-RR)

    IF Staining of K-562 using 84933-5-RR (same clone as 84933-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using YOD1 antibody (84933-5-RR, Clone: 242498G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84933-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84933-5-RR

    Affinity and Kinetic Characterization of 84933-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84933-5-RR against Human YOD1 were performed. The affinity constant is 1.07 nM.

Geprüfte Anwendungen

Produktinformation

84933-5-PBS bindet in WB, IF/ICC, Indirect ELISA YOD1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen YOD1 fusion protein Ag21844
Vollständiger Name YOD1 OTU deubiquinating enzyme 1 homolog (S. cerevisiae)
Berechnetes Molekulargewicht 348 aa, 38 kDa
Beobachtetes Molekulargewicht 38 kDa
GenBank-ZugangsnummerBC137167
Gene symbol YOD1
Gene ID (NCBI) 55432
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

YOD1, also known as YOD1 deubiquitinase, belongs to the ovarian tumor (OTU) domain-containing family of deubiquitinating enzymes (DUBs), which play a crucial role in regulating cellular processes by removing ubiquitin molecules from target proteins. YOD1 is particularly involved in the endoplasmic reticulum-associated degradation (ERAD) pathway, where it assists in the clearance of misfolded proteins from the endoplasmic reticulum (ER) by modulating the ubiquitination status of ERAD components. (PMID: 40132762,19818707)