YOD1 Rekombinanter Antikörper

YOD1 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242498G6

Kat-Nr. : 84933-5-RR

Synonyme

242498G6, DUBA 8, DUBA8, DUBA-8, EC:3.4.19.12

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  WB analysis using 84933-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84933-5-RR (YOD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of K-562 using 84933-5-RR

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using YOD1 antibody (84933-5-RR, Clone: 242498G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84933-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84933-5-RR against Human YOD1 were performed. The affinity constant is 1.07 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HEK-293-Zellen, HeLa-Zellen, Jurkat-Zellen
• Erfolgreiche Detektion in IF/ICC: K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84933-5-RR bindet in WB, IF/ICC, ELISA YOD1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: YOD1 fusion protein Ag21844
• Vollständiger Name: YOD1 OTU deubiquinating enzyme 1 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 348 aa, 38 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC137167
• Gene symbol: YOD1
• Gene ID (NCBI): 55432
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

YOD1, also known as YOD1 deubiquitinase, belongs to the ovarian tumor (OTU) domain-containing family of deubiquitinating enzymes (DUBs), which play a crucial role in regulating cellular processes by removing ubiquitin molecules from target proteins. YOD1 is particularly involved in the endoplasmic reticulum-associated degradation (ERAD) pathway, where it assists in the clearance of misfolded proteins from the endoplasmic reticulum (ER) by modulating the ubiquitination status of ERAD components. (PMID: 40132762,19818707)