YPEL5 Polyklonaler Antikörper

YPEL5 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Rezensionen (1)

Kat-Nr. : 11730-1-AP

Synonyme

CGI 127, Protein yippee like 5, yippee like 5 (Drosophila), YPEL5

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Galerie der Validierungsdaten

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  WB analysis using 11730-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11730-1-AP (YPEL5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11730-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11730-1-AP (YPEL5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11730-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11730-1-AP (YPEL5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 11730-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11730-1-AP (YPEL5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11730-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11730-1-AP (YPEL5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11730-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11730-1-AP (YPEL5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 11730-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11730-1-AP (YPEL5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 11730-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11730-1-AP (YPEL5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Maushodengewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

11730-1-AP bindet in WB, IHC, IF, ELISA YPEL5 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: YPEL5 fusion protein Ag2328
• Vollständiger Name: yippee-like 5 (Drosophila)
• Berechnetes Molekulargewicht: 121 aa, 14 kDa
• Beobachtetes Molekulargewicht: 14 kDa
• GenBank-Zugangsnummer: BC000836
• Gene symbol: YPEL5
• Gene ID (NCBI): 51646
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

YPEL5(Protein yippee-like 5) belongs to the yippee family and is involved in a certain cell division-related function.During cell cycle, YPEL5 protein is detected at different subcellular localizations; at interphase, it is located in the nucleus and centrosome, then it changes location sequentially to spindle poles, mitotic spindle, and spindle midzone during mitosis, and finally transferrs to midbody at cytokinesis(PMID:20580816).

Publikationen

Species	Application	Title
zebrafish	WB,IF	Neuron; Molecular microcircuitry underlies functional specification in a basal ganglia circuit dedicated to vocal learning.; Authors - Hilliard Austin T AT
human	WB	Mol Oncol; METTL3/YTHDF2 m6A axis accelerates colorectal carcinogenesis through epigenetically suppressing YPEL5.; Authors - Dan Zhou
human	WB	iScience; Genome-wide CRISPR-Cas9 screen analyzed by SLIDER identifies network of repressor complexes that regulate TRIM24; Authors - Lalit R Patel

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

S (Verified Customer) (11-22-2021)

The band was there on the right MW range, but it was a bit weak. It could have been better if I used 0.2 um pore-size membrane, given that YPEL5 is a small protein.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HEK293T

YPEL5 Antibody Western Blot validation (1:2000 dilution) in HEK293T (Cat no:11730-1-AP)