YTHDC1 Monoklonaler Antikörper

YTHDC1 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

1F5G11

Kat-Nr. : 67911-1-PBS

Synonyme

1F5G11, KIAA1966, Splicing factor YT521, YT521, YT521 B

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  WB analysis using 67911-1-Ig (same clone as 67911-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67911-1-Ig (YTHDC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control. This data was developed using the same antibody clone with 67911-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67911-1-Ig (same clone as 67911-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67911-1-Ig (YTHDC1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67911-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67911-1-Ig (same clone as 67911-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67911-1-Ig (YTHDC1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67911-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 67911-1-Ig (same clone as 67911-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67911-1-Ig (YTHDC1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67911-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 67911-1-Ig (same clone as 67911-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67911-1-Ig (YTHDC1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67911-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 67911-1-Ig (same clone as 67911-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 67911-1-Ig (YTHDC1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67911-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67911-1-PBS bindet in WB, IHC, ELISA YTHDC1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: YTHDC1 fusion protein Ag6289
• Vollständiger Name: YTH domain containing 1
• Berechnetes Molekulargewicht: 85 kDa
• Beobachtetes Molekulargewicht: ~100 kDa
• GenBank-Zugangsnummer: BC053863
• Gene symbol: YTHDC1
• Gene ID (NCBI): 91746
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

YTHDC1, containing 1 YTH domain, is one of the m6A-binding proteins that can recognize and bind to the m6A methylation site and plays a specific role in gene expression. YTHDC1 is constitutively enriched in the nucleus. It regulates mRNA splicing by bridging the interactions between the trans- and cis-regulatory elements to bind targeted mRNAs. YTHDC1 mediates the export of m6A modified mRNA from the nucleus to the cytoplasm by interacting with SRSF3, which is an essential factor driving the tumorigenic process in various types of cancers including breast cancer, colon cancer, ovarian cancer, osteosarcoma, and glioblastoma (32368386).