YTHDC2 Rekombinanter Antikörper

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• KD/KO Validated

YTHDC2 Rekombinant Antikörper für WB, IHC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

241123A9

Kat-Nr. : 83970-1-PBS

Synonyme

241123A9, 3'-5' RNA helicase YTHDC2, EC:3.6.4.13, hYTHDC2, YTH domain-containing protein 2

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  WB analysis using 83970-1-RR (same clone as 83970-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83970-1-RR (YTHDC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83970-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 83970-1-RR (same clone as 83970-1-PBS)

  WB result of YTHDC2 antibody (83970-1-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-YTHDC2 transfected HeLa cells. This data was developed using the same antibody clone with 83970-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP00893-3

  Sandwich ELISA standard curve of MP00893-3, Human YTHDC2 Recombinant Matched Antibody Pair - PBS only. 83970-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag26690. 83970-1-PBS was HRP conjugated as the detection antibody. Range: 0.625-20 ng/mL

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  Sample test of MP00893-3

  The mean YTHDC2 concentration was determined to be 4.89 ng/mL in HEK-293T cell extract based on a 1.4 mg/mL extract load.

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  IHC staining of rat testis using 83970-1-RR (same clone as 83970-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 83970-1-RR (YTHDC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83970-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 83970-1-RR (same clone as 83970-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 83970-1-RR (YTHDC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83970-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 83970-1-RR (same clone as 83970-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug YTHDC2 Recombinant antibody (83970-1-RR, Clone:241123A9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83970-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83970-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83970-1-RR against Human YTHDC2 were performed. The affinity constant is 0.378 nM.

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Geprüfte Anwendungen

Produktinformation

83970-1-PBS bindet in WB, IHC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test YTHDC2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: YTHDC2 fusion protein Ag26690
• Vollständiger Name: YTH domain containing 2
• Beobachtetes Molekulargewicht: 160 kDa
• GenBank-Zugangsnummer: BC137285
• Gene symbol: YTHDC2
• Gene ID (NCBI): 64848
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

YTHDC2, also named as Probable ATP-dependent RNA helicase YTHDC2, is a 1430 amino acid protein, which belongs to the DEAD box helicase family. YTHDC2 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability.