YTHDF1 Monoklonaler Antikörper

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YTHDF1 Monoklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3A2H12

Kat-Nr. : 66745-1-PBS

Synonyme

3A2H12, C20orf21, DACA-1, DF1, YTH domain-containing family protein 1

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  WB analysis of HepG2 using 66745-1-Ig (same clone as 66745-1-PBS)

  WB result of YTHDF1 antibody (66745-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YTHDF1 transfected HepG2 cells. This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

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  WB analysis using 66745-1-Ig (same clone as 66745-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66745-1-Ig (YTHDF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

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  IP experiment of Jurkat using 66745-1-Ig (same clone as 66745-1-PBS)

  IP result of anti-YTHDF1 (IP:66745-1-Ig, 4ug; Detection:66745-1-Ig 1:2000) with Jurkat cells lysate 2000 ug. This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66745-1-Ig (same clone as 66745-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66745-1-Ig (YTHDF1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 66745-1-Ig (same clone as 66745-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66745-1-Ig (YTHDF1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66745-1-Ig (same clone as 66745-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66745-1-Ig (YTHDF1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66745-1-Ig (same clone as 66745-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66745-1-Ig (YTHDF1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 66745-1-Ig (same clone as 66745-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66745-1-Ig (YTHDF1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 66745-1-Ig (same clone as 66745-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66745-1-Ig (YTHDF1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66745-1-Ig (same clone as 66745-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66745-1-Ig (YTHDF1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66745-1-Ig (same clone as 66745-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66745-1-Ig (YTHDF1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

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  IHC staining of rat testis using 66745-1-Ig (same clone as 66745-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 66745-1-Ig (YTHDF1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat testis using 66745-1-Ig (same clone as 66745-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 66745-1-Ig (YTHDF1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 66745-1-Ig (same clone as 66745-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66745-1-Ig (YTHDF1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66745-1-PBS bindet in WB, IHC, IP, Indirect ELISA YTHDF1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: YTHDF1 fusion protein Ag11633
• Vollständiger Name: YTH domain family, member 1
• Berechnetes Molekulargewicht: 559 aa, 61 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC050284
• Gene symbol: YTHDF1
• Gene ID (NCBI): 54915
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

YTHDF1, also named as YTH domain-containing family protein 1 or C20orf21, is a 559 amino acid protein, which localizes in the cytoplasm. YTHDF1 specifically recognizes and binds N6-methyladenosine (m6A)-containing mRNAs, and promotes mRNA translation efficiency. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. YTHDF1 acts as a regulator of mRNA translation efficiency: promotes ribosome loading to m6A-containing mRNAs and interacts with translation initiation factors eIF3 (EIF3A or EIF3B) to facilitate translation initiation. YTHDF1 exists two isoform and calculated molecular weight of isoforms are 61 kDa and 21 kDa.