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  • Featured Product
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YTHDF2 Rekombinanter Antikörper

YTHDF2 Rekombinant Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

3L6

Kat-Nr. : 81340-1-RR

Synonyme

3L6, CLL associated antigen KW 14, CLL-associated antigen KW-14, DF2, HGRG8

Filter:
  • Western Blot (WB) analysis of various lysates using YTHDF2 Recombinant antibody (81340-1-RR)

    WB analysis using 81340-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81340-1-RR (YTHDF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using YTHDF2 Recombinant antibody (81340-1-RR)

    WB analysis of Jurkat using 81340-1-RR

    WB result of YTHDF2 antibody (81340-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YTHDF2 transfected Jurkat cells.

  • Immunoprecipitation (IP) experiment of HeLa cells using YTHDF2 Recombinant antibody (81340-1-RR)

    IP experiment of HeLa using 81340-1-RR

    IP result of anti-YTHDF2 (IP:81340-1-RR, 4ug; Detection:81340-1-RR 1:1000) with HeLa cells lysate 1880 ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using YTHDF2 Recombinant antibody (81340-1-RR)

    IF Staining of HeLa using 81340-1-RR

    Immunofluorescent analysis of (4% PFA) fixed sodium arsenite treated HeLa cells using YTHDF2 antibody (81340-1-RR, Clone: 3L6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 81340-1-RR

    Affinity and Kinetic Characterization of 81340-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81340-1-RR against Human YTHDF2 were performed. The affinity constant is 41.6 pM.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBHEK-293-Zellen, C6-Zellen, HeLa-Zellen, Jurkat-Zellen, NIH/3T3-Zellen, Raji-Zellen, RAW 264.7-Zellen
Erfolgreiche IPHeLa-Zellen
Erfolgreiche Detektion in IF/ICCsodium arsenite treated HeLa cells

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:10000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

81340-1-RR bindet in WB, IF/ICC, IP, ELISA YTHDF2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen YTHDF2 fusion protein Ag5922
Vollständiger Name YTH domain family, member 2
Berechnetes Molekulargewicht 62 kDa
Beobachtetes Molekulargewicht 65-70 kDa
GenBank-ZugangsnummerBC002559
Gene symbol YTHDF2
Gene ID (NCBI) 51441
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

YTHDF2, also named as YTH domain-containing family protein 2, is a 579 amino acid protein, which contains 1 YTH domain. YTHDF2 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, and regulates mRNA stability. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. YTHDF2 acts as a regulator of mRNA stability: binding to m6A-containing mRNAs results in the localization to mRNA decay sites, such as processing bodies (P-bodies), leading to mRNA degradation. YTHDF2 also acts as a promoter of cap-independent mRNA translation following heat shock stress: upon stress, relocalizes to the nucleus and specifically binds mRNAs with some m6A methylation mark at their 5'-UTR, protecting demethylation of mRNAs by FTO, thereby promoting cap-independent mRNA translation. The calculated molecular weight of YTHDF2 is 62 kDa, but the phosphorylated YTHDF2 is about 65-70 kDa.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for YTHDF2 antibody 81340-1-RRProtokoll herunterladen
IF protocol for YTHDF2 antibody 81340-1-RRProtokoll herunterladen
IP protocol for YTHDF2 antibody 81340-1-RRProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Nikolett (Verified Customer) (07-23-2025)

Antibody works well as 1:1000 for Western Blot, 1:300 for IHC and IF. For Western Blot I used various human lung cancer cell lines. For IHC and IF I used human NSCLC patient samples.

  • Applications: Western Blot, Immunohistochemistry, Immunofluorescence
  • Primary Antibody Dilution: 1:1000 for Western Blot, 1:300 for IHC and IF
  • Cell Tissue Type: Human Lung Cancer Patient Samples