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YTHDF2 Rekombinanter Antikörper
YTHDF2 Rekombinant Antikörper für WB, IF/ICC, IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte
Anwendung
WB, IF/ICC, IP, ELISA
Konjugation
Unkonjugiert
CloneNo.
3L6
Kat-Nr. : 81340-1-RR
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | HEK-293-Zellen, C6-Zellen, HeLa-Zellen, Jurkat-Zellen, NIH/3T3-Zellen, Raji-Zellen, RAW 264.7-Zellen | 
| Erfolgreiche IP | HeLa-Zellen | 
| Erfolgreiche Detektion in IF/ICC | sodium arsenite treated HeLa cells | 
Empfohlene Verdünnung
| Anwendung | Verdünnung | 
|---|---|
| Western Blot (WB) | WB : 1:5000-1:10000 | 
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate | 
| Immunfluoreszenz (IF)/ICC | IF/ICC : 1:200-1:800 | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Produktinformation
81340-1-RR bindet in WB, IF/ICC, IP, ELISA YTHDF2 und zeigt Reaktivität mit human, Maus, Ratten
| Getestete Reaktivität | human, Maus, Ratte | 
| Wirt / Isotyp | Kaninchen / IgG | 
| Klonalität | Rekombinant | 
| Typ | Antikörper | 
| Immunogen | YTHDF2 fusion protein Ag5922 | 
| Vollständiger Name | YTH domain family, member 2 | 
| Berechnetes Molekulargewicht | 62 kDa | 
| Beobachtetes Molekulargewicht | 65-70 kDa | 
| GenBank-Zugangsnummer | BC002559 | 
| Gene symbol | YTHDF2 | 
| Gene ID (NCBI) | 51441 | 
| Konjugation | Unkonjugiert | 
| Form | Liquid | 
| Reinigungsmethode | Protein-A-Reinigung | 
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol | 
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. | 
Hintergrundinformationen
YTHDF2, also named as YTH domain-containing family protein 2, is a 579 amino acid protein, which contains 1 YTH domain. YTHDF2 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, and regulates mRNA stability. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. YTHDF2 acts as a regulator of mRNA stability: binding to m6A-containing mRNAs results in the localization to mRNA decay sites, such as processing bodies (P-bodies), leading to mRNA degradation. YTHDF2 also acts as a promoter of cap-independent mRNA translation following heat shock stress: upon stress, relocalizes to the nucleus and specifically binds mRNAs with some m6A methylation mark at their 5'-UTR, protecting demethylation of mRNAs by FTO, thereby promoting cap-independent mRNA translation. The calculated molecular weight of YTHDF2 is 62 kDa, but the phosphorylated YTHDF2 is about 65-70 kDa.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for YTHDF2 antibody 81340-1-RR | Protokoll herunterladen | 
| IF protocol for YTHDF2 antibody 81340-1-RR | Protokoll herunterladen | 
| IP protocol for YTHDF2 antibody 81340-1-RR | Protokoll herunterladen | 
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen | 
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
| FH Nikolett (Verified Customer) (07-23-2025) | Antibody works well as 1:1000 for Western Blot, 1:300 for IHC and IF. For Western Blot I used various human lung cancer cell lines. For IHC and IF I used human NSCLC patient samples. 
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