YTHDF3-specific Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

YTHDF3-specific Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, RIP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

68

Rezensionen (1)

Kat-Nr. : 25537-1-AP

Synonyme

YTH domain family protein 3, YTH domain family, member 3, YTHDF3, YTHDF3-specific

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 25537-1-AP

  WB result of YTHDF3-specific antibody (25537-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YTHDF3-specific transfected HeLa cells.

• 

  WB analysis using 25537-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25537-1-AP (YTHDF3-specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of A549 using 25537-1-AP

  Immunofluorescent analysis of A549 cells using 25537-1-AP (YTHDF3-specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HCT 116-Zellen, HeLa-Zellen, HepG2-Zellen, NIH/3T3-Zellen, PC-12-Zellen
• Erfolgreiche Detektion in IF: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 18 publications below
• WB: See 59 publications below
• IHC: See 5 publications below
• IF: See 8 publications below
• CoIP: See 2 publications below
• RIP: See 10 publications below

Produktinformation

25537-1-AP bindet in WB, RIP, IHC, IF, CoIP, ELISA YTHDF3-specific und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Huhn, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: YTHDF3-specific fusion protein Ag22374
• Vollständiger Name: YTH domain family, member 3
• Berechnetes Molekulargewicht: 585 aa, 64 kDa
• Beobachtetes Molekulargewicht: 64 kDa
• GenBank-Zugangsnummer: BC052970
• Gene symbol: YTHDF3
• Gene ID (NCBI): 253943
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

YTHDF3, also named as YTH domain-containing family protein 3, is a 585 amino acid protein, which contains 1 YTH domain. YTHDF3 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. Our antibody 25537-1-AP specially reacts with YTHDF3 protein.

Publikationen

Species	Application	Title
mouse	WB	Nature; m6A facilitates hippocampus-dependent learning and memory through YTHDF1.; Authors - Hailing Shi
human	WB,CoIP	Mol Cancer; A positive feedback circuit driven by m6A-modified circular RNA facilitates colorectal cancer liver metastasis; Authors - Kaixuan Zeng ; KD Validated
human	WB	Cell Res; Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells.; Authors - Fei Peng
human	WB	Nat Commun; Circular RNA encoded MET variant promotes glioblastoma tumorigenesis; Authors - Jian Zhong ; KD Validated
human	WB	Genes Dev; PMID: 30463905; Authors - Rosa M Rubio
human	IF	Nat Chem Biol; m6A-binding YTHDF proteins promote stress granule formation.; Authors - Ye Fu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Robert (Verified Customer) (01-17-2019)

1. scrambled siRNA2. YTHDF3 siRNA

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: ST cells (porcine)

YTHDF3-specific Antibody Western Blot, validation (1/1000 dilution) in ST cells (porcine) (Cat no:25537-1-AP)