YTHDF3 Rekombinanter Antikörper

YTHDF3 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

242149F3

Kat-Nr. : 84709-3-RR

Synonyme

YTH domain-containing family protein 3, DF3, 242149F3

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  WB analysis using 84709-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84709-3-RR (YTHDF3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84709-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84709-3-RR against Human YTHDF3 were performed. The affinity constant is 0.18 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HCT 116-Zellen, C6-Zellen, NIH/3T3-Zellen, PC-12-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84709-3-RR bindet in WB, ELISA YTHDF3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: YTHDF3 fusion protein Ag22407
• Vollständiger Name: YTH domain family, member 3
• Berechnetes Molekulargewicht: 585 aa, 64 kDa
• Beobachtetes Molekulargewicht: 64 kDa
• GenBank-Zugangsnummer: BC052970
• Gene symbol: YTHDF3
• Gene ID (NCBI): 253943
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

YTHDF3, also named as YTH domain-containing family protein 3, is a 585 amino acid protein, which contains 1 YTH domain. YTHDF3 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability.