YWHAB Polyklonaler Antikörper

YWHAB Polyklonal Antikörper für IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10936-1-PBS

Synonyme

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  IHC staining of human colon cancer using 10936-1-AP (same clone as 10936-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10936-1-AP (YWHAB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10936-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 10936-1-AP (same clone as 10936-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10936-1-AP (YWHAB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10936-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 10936-1-AP (same clone as 10936-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10936-1-AP (YWHAB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10936-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 10936-1-AP (same clone as 10936-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10936-1-AP (YWHAB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10936-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 10936-1-AP (same clone as 10936-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using YWHAB antibody (10936-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 10936-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10936-1-PBS bindet in IHC, IF/ICC, Indirect ELISA YWHAB und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: YWHAB fusion protein Ag1383
• Vollständiger Name: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta polypeptide
• Berechnetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC010352
• Gene symbol: YWHAB
• Gene ID (NCBI): 7529
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

YWHAB (also known as 14-3-3 beta) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis. This antibody was raised agaist the N-terminal region of human YWHAB.