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YWHAH Rekombinanter Antikörper

YWHAH Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250473G10

Kat-Nr. : 85987-2-PBS

Synonyme

14-3-3 eta, 14 3 3 protein eta, 14-3-3, 14-3-3 protein eta

Filter:
  • Western Blot (WB) analysis of various lysates using YWHAH Recombinant monoclonal antibody (85987-2-RR)

    WB analysis using 85987-2-RR (same clone as 85987-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85987-2-RR (YWHAH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85987-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using YWHAH Recombinant monoclonal antibody (85987-2-RR)

    IF Staining of HeLa using 85987-2-RR (same clone as 85987-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using YWHAH antibody (85987-2-RR, Clone: 250473G10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85987-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85987-2-RR

    Affinity and Kinetic Characterization of 85987-2-RR

    Biolayer interferometry (BLl) kinetic assays of 85987-2-RR against Human YWHAH were performed. The affinity constant is below 1 pM.

Geprüfte Anwendungen

Produktinformation

85987-2-PBS bindet in WB, IF/ICC, Indirect ELISA YWHAH und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen YWHAH fusion protein Ag7435
Vollständiger Name tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, eta polypeptide
Berechnetes Molekulargewicht 28 kDa
Beobachtetes Molekulargewicht 28 kDa
GenBank-ZugangsnummerBC003047
Gene symbol YWHAH
Gene ID (NCBI) 7533
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

YWHAH (also known as 14-3-3 eta) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis. This antibody was raised against the full-length YWHAH.