YWHAZ Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

YWHAZ Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

19

Rezensionen (1)

Kat-Nr. : 14881-1-AP

Synonyme

14 3 3 protein zeta/delta, 14-3-3, KCIP 1, YWHAZ

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Galerie der Validierungsdaten

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  WB analysis using 14881-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14881-1-AP (YWHAZ antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14881-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14881-1-AP (YWHAZ antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14881-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14881-1-AP (YWHAZ antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14881-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14881-1-AP (YWHAZ antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, Jurkat-Zellen, Maushirngewebe, NIH/3T3-Zellen, Raji-Zellen, Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 15 publications below
• IHC: See 4 publications below
• IF: See 1 publications below
• CoIP: See 2 publications below

Produktinformation

14881-1-AP bindet in WB, IHC, IF, CoIP, ELISA YWHAZ und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: YWHAZ fusion protein Ag6654
• Vollständiger Name: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide
• Berechnetes Molekulargewicht: 28 kDa
• Beobachtetes Molekulargewicht: 28-30 kDa
• GenBank-Zugangsnummer: BC003623
• Gene symbol: YWHAZ
• Gene ID (NCBI): 7534
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

YWHAZ (also known as 14-3-3 zeta) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis. This antibody was raised against the full-length YWHAZ.

Publikationen

Species	Application	Title
mouse	WB	Mol Cell; Itaconate is a lysosomal inducer that promotes antibacterial innate immunity.; Authors - Zhenxing Zhang
mouse	WB	EMBO J; AKT2 reduces IFNβ1 production to modulate antiviral responses and systemic lupus erythematosus.; Authors - Xin Zheng
human	WB,IHC	Oncogene; Lysine-specific demethylase 1 promotes the stemness and chemoresistance of Lgr5(+) liver cancer initiating cells by suppressing negative regulators of β-catenin signaling.; Authors - Z-J Lei ; KD Validated
		Oncogene; miR-155 downregulates ErbB2 and suppresses ErbB2-induced malignant transformation of breast epithelial cells.; Authors - X-H He
human	WB	EMBO Rep; CUE domain-containing protein 2 promotes the Warburg effect and tumorigenesis.; Authors - Xiuying Zhong ; KD Validated
human	WB	Haematologica; Comparative analysis of ChAdOx1 nCoV-19 and Ad26.COV2.S SARS-CoV-2 vector vaccines.; Authors - Stephan Michalik

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Erik (Verified Customer) (08-26-2021)

We used the antibody at 1:1000 dilution with 11ug input protein, and it produced one clean and strong band at the expected height for the protein

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MIAPACA2 and PANC1 cell lines

YWHAZ Antibody Western Blot validation (1:1000 dilution) in MIAPACA2 and PANC1 cell lines (Cat no:14881-1-AP)