YY1 Polyklonaler Antikörper

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• KD/KO Validated

YY1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (3)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, chIP, ELISA

Konjugation

Unkonjugiert

Publikationen(46)

Kat-Nr. : 22156-1-AP

Synonyme

UCRBP, NF-E1, INO80S, INO 80S, Delta transcription factor

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• Validation Data Gallery
• Proteintech vs. Other Vendors

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  WB analysis of Jurkat using 22156-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22156-1-AP (YY1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 cells using 22156-1-AP

  A549 cells (shcontrol and shRNA of YY1) were subjected to SDS PAGE followed by western blot with 22156-1-AP (YY1 antibody) at dilution of 1:1000.

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  WB analysis using 22156-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22156-1-AP (YY1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 22156-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22156-1-AP (YY1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 22156-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22156-1-AP (YY1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 22156-1-AP

  IP result of anti-YY1 (IP:22156-1-AP, 5ug; Detection:22156-1-AP 1:700) with HeLa cells lysate 880ug.

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  IHC staining of human breast cancer using 22156-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22156-1-AP (YY1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 22156-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22156-1-AP (YY1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 22156-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using YY1 antibody (22156-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, A549-Zellen, HEK-293-Zellen, HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 9 publications below
• WB: See 31 publications below
• IHC: See 4 publications below
• IF: See 9 publications below
• IP: See 3 publications below
• CoIP: See 4 publications below
• ChIP: See 12 publications below

Produktinformation

22156-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, chIP, ELISA YY1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: YY1 fusion protein Ag17792
• Vollständiger Name: YY1 transcription factor
• Berechnetes Molekulargewicht: 414 aa, 45 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC037308
• Gene symbol: YY1
• Gene ID (NCBI): 7528
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

YY1, also named as DELTA, INO80S and NF-E1, contains four C2H2-type zinc fingers and belongs to the YY transcription factor family. YY1 is a multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the YY1. The open reading frame of the human YY1 cDNA encodes a protein of 414 amino acids with a predicted molecular weight of 44 kDa. However, YY1 migrates on SDS gels as a 65-68 kDa protein, probably due to the structure of the protein. It is a ubiquitously expressed transcription factor with fundamental roles in embryogenesis, differentiation, replication and proliferation. This is a rabbit polyclonal antibody raised against full length YY1 of human origin.

Publikationen

Species	Application	Title
human	IF	Sci Transl Med; Yin Yang 1 protein ameliorates diabetic nephropathy pathology through transcriptional repression of TGFβ1.; Authors - Pan Gao
human	WB	Nat Commun; Mutant huntingtin induces neuronal apoptosis via derepressing the non-canonical poly(A) polymerase PAPD5; Authors - Zhefan Stephen Chen
human	WB	Mol Cell; Dynamic control of chromatin-associated m6A methylation regulates nascent RNA synthesis.; Authors - Wenqi Xu
human	WB	Theranostics; KDM6A promotes imatinib resistance through YY1-mediated transcriptional upregulation of TRKA independently of its demethylase activity in chronic myelogenous leukemia.; Authors - Chengwan Zhang ; KD Validated
human	ChIP	Cancer Lett; MORTALIN-Ca2+ axis drives innate rituximab resistance in diffuse large B-cell lymphoma.; Authors - Qi Sun
human	WB,IP,ChIP	Free Radic Biol Med; Asymmetrical arginine dimethylation of histone H4 by 8-oxog/OGG1/PRMT1 is essential for oxidative stress-induced transcription activation.; Authors - Wentao Wang