ZAK Polyklonaler Antikörper

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• KD/KO Validated

ZAK Polyklonal Antikörper für WB, IF, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(10)

Rezensionen (1)

Kat-Nr. : 14945-1-AP

Synonyme

AZK, HCCS 4, MAP3K20, Mitogen-activated protein kinase kinase kinase 20, MLK related kinase, MLK7, mlklak, MLT, MLTK, MRK, ZAK

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  WB analysis using 14945-1-AP

  mouse skeletal muscle lysates were subjected to SDS PAGE followed by western blot with 14945-1-AP (ZAK antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14945-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14945-1-AP (ZAK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 14945-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14945-1-AP (ZAK antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 14945-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14945-1-AP (ZAK antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 14945-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14945-1-AP (ZAK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14945-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14945-1-AP (ZAK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14945-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14945-1-AP (ZAK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 14945-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14945-1-AP (ZAK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 14945-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14945-1-AP (ZAK Antibody) at dilution of 1:50(under 10x lens).

• 

  IHC staining of human placenta using 14945-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14945-1-AP (ZAK Antibody) at dilution of 1:50(under 40x lens).

• 

  IF Staining of HepG2 using 14945-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ZAK antibody (14945-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe, HepG2-Zellen, humanes Herzgewebe, humanes Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1500
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 9 publications below

Produktinformation

14945-1-AP bindet in WB, IHC, IF/ICC, ELISA ZAK und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZAK fusion protein Ag6747
• Vollständiger Name: sterile alpha motif and leucine zipper containing kinase AZK
• Berechnetes Molekulargewicht: 91 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC001401
• Gene symbol: ZAK
• Gene ID (NCBI): 51776
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ZAK(sterile-alpha motif and leucine zipper containing kinase AZK) is also named as MLTK, MAPKKK, mlklak, MLK7, AZK, MLT, MRK, HCCS-4 and belongs to the MAPKKK family. It is a mitogen-activated protein kinase kinase kinase (MAP3K) that activates the stress-activated protein kinase/c-jun N-terminal kinase pathway and activates NF-kappaB. ZAK contributes to regulation of DNA damage checkpoints through a p38gamma-independent pathway. This protein has 3 isoforms produced by alternative splicing with the MW of 91 kDa, 51 kDa and 35 kDa.

Publikationen

Species	Application	Title
human	WB	Immunity; Cytoplasmic DNA sensing by KU complex in aged CD4+ T cell potentiates T cell activation and aging-related autoimmune inflammation.; Authors - Yan Wang ; KO Validated
human	WB	Sci Signal; Poly-GR repeats associated with ALS/FTD gene C9ORF72 impair translation elongation and induce a ribotoxic stress response in neurons; Authors - Daoyuan Dong
human	WB	bioRxiv; Initiation of a ZAKα-dependent Ribotoxic Stress Response by the Innate Immunity Endoribonuclease RNase L; Authors - Jiajia Xi
		PNAS Nexus; Ribosome stalling during c-myc translation presents actionable cancer cell vulnerability; Authors - Tejinder Pal Khaket
human	WB	Mol Cell; The ribotoxic stress response drives acute inflammation, cell death, and epidermal thickening in UV-irradiated skin in vivo; Authors - Anna Constance Vind
mouse	WB	Nat Commun; Ribosome impairment regulates intestinal stem cell identity via ZAKɑ activation; Authors - Joana Silva

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Amy (Verified Customer) (08-14-2019)

Worked well with 5% milk in 0.2% PBST.Shows up well the beta isoform in mouse muscle, and alpha in heart.Did not work in Zebrafish despite the kinase domain being highly conserved between organisms.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: C2C12 and mouse skeletal muscle tissues