ZAK Polyklonaler Antikörper

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• KD/KO Validated

ZAK Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 28761-1-AP

Synonyme

MAP3K20, Leucine zipper- and sterile alpha motif-containing kinase, HCCS-4, HCCS 4, EC:2.7.11.25

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  WB analysis using 28761-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28761-1-AP (ZAK antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 28761-1-AP

  WB result of ZAK antibody (28761-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZAK transfected HeLa cells.

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  IHC staining of human colon cancer using 28761-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28761-1-AP (ZAK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 28761-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 28761-1-AP (ZAK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse tongue using 28761-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 28761-1-AP (ZAK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat tongue using 28761-1-AP

  Immunohistochemical analysis of paraffin-embedded rat tongue tissue slide using 28761-1-AP (ZAK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 28761-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ZAK antibody (28761-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, MDA-MB-231-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, Mauszungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Produktinformation

28761-1-AP bindet in WB, IHC, IF/ICC, ELISA ZAK und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZAK fusion protein Ag30599
• Vollständiger Name: sterile alpha motif and leucine zipper containing kinase AZK
• Berechnetes Molekulargewicht: 91 kDa
• Beobachtetes Molekulargewicht: 91kDa, 51 kDa
• GenBank-Zugangsnummer: BC001401
• Gene symbol: ZAK
• Gene ID (NCBI): 51776
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ZAK(sterile-alpha motif and leucine zipper containing kinase AZK) is also named as MLTK, MAPKKK, mlklak, MLK7, AZK, MLT, MRK, HCCS-4, MAP3K20 and belongs to the MAPKKK family. It is a mitogen-activated protein kinase kinase kinase (MAP3K) that activates the stress-activated protein kinase/c-jun N-terminal kinase pathway and activates NF-kappaB. ZAK contributes to regulation of DNA damage checkpoints through a p38gamma-independent pathway. This protein has 3 isoforms produced by alternative splicing with the MW of 91 kDa, 51 kDa and 35 kDa.

Publikationen

Species	Application	Title
human	WB	Genome Biol; The collateral activity of RfxCas13d can induce lethality in a RfxCas13d knock-in mouse model; Authors - Yunfei Li ; KD Validated
human	WB	J Cell Mol Med; Linoleic Acid Enhances Renal Tubular Epithelial Cells Autophagy Caused by Calcium Oxalate Monohydrate Crystals; Authors - Weiming Ma
human,mouse	WB,IF	Inflammation; ZAKα Induces Pyroptosis of Colonic Epithelium Via the Caspase-11/GSDMD Pathway to Aggravate Colitis; Authors - Song Li