ZAK Monoklonaler Antikörper

ZAK Monoklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Kaninchen, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2H10E4

Kat-Nr. : 68599-1-PBS

Synonyme

2H10E4, AZK, EC:2.7.11.25, HCCS 4, HCCS-4

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  WB analysis using 68599-1-Ig (same clone as 68599-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68599-1-Ig (ZAK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68599-1-PBS in a different storage buffer formulation.

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  WB analysis of rat skeletal muscle using 68599-1-Ig (same clone as 68599-1-PBS)

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 68599-1-Ig (ZAK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68599-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rabbit skeletal muscle using 68599-1-Ig (same clone as 68599-1-PBS)

  rabbit skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 68599-1-Ig (ZAK antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68599-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68599-1-PBS bindet in WB, Indirect ELISA ZAK und zeigt Reaktivität mit human, Kaninchen, Ratten

• Getestete Reaktivität: human, Kaninchen, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ZAK fusion protein Ag6747
• Vollständiger Name: sterile alpha motif and leucine zipper containing kinase AZK
• Berechnetes Molekulargewicht: 91 kDa
• Beobachtetes Molekulargewicht: 91 kDa
• GenBank-Zugangsnummer: BC001401
• Gene symbol: ZAK
• Gene ID (NCBI): 51776
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ZAK(sterile-alpha motif and leucine zipper containing kinase AZK) is also named as MLTK, MAPKKK, mlklak, MLK7, AZK, MLT, MRK, HCCS-4 and belongs to the MAPKKK family. It is a mitogen-activated protein kinase kinase kinase (MAP3K) that activates the stress-activated protein kinase/c-jun N-terminal kinase pathway and activates NF-kappaB. ZAK contributes to regulation of DNA damage checkpoints through a p38gamma-independent pathway. This protein has 3 isoforms produced by alternative splicing with the MW of 91 kDa, 51 kDa and 35 kDa.