ZAK Rekombinanter Antikörper

ZAK Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

250559E6

Kat-Nr. : 81161-2-RR

Synonyme

AZK, EC:2.7.11.25, HCCS 4, HCCS-4, Leucine zipper- and sterile alpha motif-containing kinase

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  WB analysis using 81161-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81161-2-RR (ZAK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 81161-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 81161-2-RR (ZAK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 81161-2-RR

  Biolayer interferometry (BLl) kinetic assays of 81161-2-RR against Human ZAK were performed. The affinity constant is 0.28 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, MDA-MB-231-Zellen, Maus-Skelettmuskelgewebe, Ratten-Skelettmuskelgewebe, U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

81161-2-RR bindet in WB, ELISA ZAK und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ZAK fusion protein Ag30599
• Vollständiger Name: sterile alpha motif and leucine zipper containing kinase AZK
• Berechnetes Molekulargewicht: 91 kDa
• Beobachtetes Molekulargewicht: 91kDa, 51 kDa
• GenBank-Zugangsnummer: BC001401
• Gene symbol: ZAK
• Gene ID (NCBI): 51776
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ZAK(sterile-alpha motif and leucine zipper containing kinase AZK) is also named as MLTK, MAPKKK, mlklak, MLK7, AZK, MLT, MRK, HCCS-4, MAP3K20 and belongs to the MAPKKK family. It is a mitogen-activated protein kinase kinase kinase (MAP3K) that activates the stress-activated protein kinase/c-jun N-terminal kinase pathway and activates NF-kappaB. ZAK contributes to regulation of DNA damage checkpoints through a p38 gamma-independent pathway. This protein has 3 isoforms produced by alternative splicing with the MW of 91 kDa (ZAK alpha), 51 kDa (ZAK beta) and 35 kDa.