ZAK Rekombinanter Antikörper

ZAK Rekombinant Antikörper für IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250559E1

Kat-Nr. : 81161-3-RR

Synonyme

AZK, EC:2.7.11.25, HCCS 4, HCCS-4, Leucine zipper- and sterile alpha motif-containing kinase

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  IHC staining of human liver cancer using 81161-3-RR

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81161-3-RR (ZAK antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 81161-3-RR

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 81161-3-RR (ZAK antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 81161-3-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using ZAK antibody (81161-3-RR, Clone: 250559E1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 81161-3-RR

  Biolayer interferometry (BLl) kinetic assays of 81161-3-RR against Human ZAK were performed. The affinity constant is 2.57 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

81161-3-RR bindet in IHC, IF/ICC, ELISA ZAK und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ZAK fusion protein Ag30599
• Vollständiger Name: sterile alpha motif and leucine zipper containing kinase AZK
• Berechnetes Molekulargewicht: 91 kDa
• GenBank-Zugangsnummer: BC001401
• Gene symbol: ZAK
• Gene ID (NCBI): 51776
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ZAK(sterile-alpha motif and leucine zipper containing kinase AZK) is also named as MLTK, MAPKKK, mlklak, MLK7, AZK, MLT, MRK, HCCS-4, MAP3K20 and belongs to the MAPKKK family. It is a mitogen-activated protein kinase kinase kinase (MAP3K) that activates the stress-activated protein kinase/c-jun N-terminal kinase pathway and activates NF-kappaB. ZAK contributes to regulation of DNA damage checkpoints through a p38 gamma-independent pathway. This protein has 3 isoforms produced by alternative splicing with the MW of 91 kDa (ZAK alpha), 51 kDa (ZAK beta) and 35 kDa.