ZAP70 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ZAP70 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Rezensionen (2)

Kat-Nr. : 15592-1-AP

Synonyme

70 kDa zeta associated protein, 70 kDa zeta-chain associated protein, EC:2.7.10.2, SRK, STD

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  WB analysis of Jurkat using 15592-1-AP

  WB result of ZAP70 antibody (15592-1-AP, 1:20000) with si-control and si-ZAP70 transfected Jurkat cells.

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  WB analysis using 15592-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15592-1-AP (ZAP70 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 15592-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 15592-1-AP (ZAP70 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 15592-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15592-1-AP (ZAP70 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 15592-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 15592-1-AP (ZAP70 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 15592-1-AP

  IP result of anti-ZAP70 (IP:15592-1-AP, 4ug; Detection:15592-1-AP 1:1000) with Jurkat cells lysate 3200ug.

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  IHC staining of human tonsillitis using 15592-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 15592-1-AP (ZAP70 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human tonsillitis using 15592-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 15592-1-AP (ZAP70 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lymphoma using 15592-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 15592-1-AP (ZAP70 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lymphoma using 15592-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 15592-1-AP (ZAP70 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 15592-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15592-1-AP (ZAP70 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 15592-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15592-1-AP (ZAP70 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 15592-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15592-1-AP (ZAP70 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 15592-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15592-1-AP (ZAP70 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of RAW 264.7 using 15592-1-AP

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using ZAP70 antibody (15592-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, A375-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Nierengewebe, humanes Lymphomgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:20000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

15592-1-AP bindet in WB, IHC, IP, ELISA ZAP70 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZAP70 fusion protein Ag7943
• Vollständiger Name: zeta-chain (TCR) associated protein kinase 70kDa
• Berechnetes Molekulargewicht: 70 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC039039
• Gene symbol: ZAP70
• Gene ID (NCBI): 7535
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Zeta-associated protein (ZAP)-70, a member of the Syk family of tyrosine kinases, plays an important role in T-cell receptor signaling, natural killer (NK) cell activation and early B-cell development. ZAP-70 is expressed in a subset of cases of chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) with unmutated immunoglobulin heavy-chain variable region (IgVH) genes and is associated with poor clinical outcome.

Publikationen

Species	Application	Title
human	WB	J Immunol Methods; An optimized non-T cell transfection system based on HEK293FT cells for CD3ζ phosphorylation and ubiquitination; Authors - Jiaqi Zheng

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Titouan (Verified Customer) (01-31-2025)

Works very well

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Jurkat

ZAP70 Antibody Western Blot validation (1:1000 dilution) in Jurkat (Cat no:15592-1-AP)

FH

hala (Verified Customer) (01-27-2023)

works very good

• Applications: Western Blot
• Primary Antibody Dilution: 1/10000
• Cell Tissue Type: hepG2

ZAP70 Antibody Western Blot validation (1/10000 dilution) in hepG2 (Cat no:15592-1-AP)