ZBP1 Polyklonaler Antikörper

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• KD/KO Validated

ZBP1 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13285-1-PBS

Synonyme

C20orf183, DAI, DLM 1, DLM1, Tumor stroma and activated macrophage protein DLM-1

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  WB analysis using 13285-1-AP (same clone as 13285-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 13285-1-AP (ZBP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13285-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 13285-1-AP (same clone as 13285-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13285-1-AP (ZBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13285-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 13285-1-AP (same clone as 13285-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13285-1-AP (ZBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13285-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 13285-1-AP (same clone as 13285-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13285-1-AP (ZBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13285-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 13285-1-AP (same clone as 13285-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ZBP1 antibody (13285-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 13285-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

13285-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA ZBP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ZBP1 fusion protein Ag4119
• Vollständiger Name: Z-DNA binding protein 1
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 42-68 kDa
• GenBank-Zugangsnummer: BC028218
• Gene symbol: ZBP1
• Gene ID (NCBI): 81030
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ZBP1(Z-DNA-binding protein 1), also named as DAI or DLM-1, is a nucleic acid sensor with some isoforms. It has been reported that ZBP1 can sense the viral nucleic acids of viruses and induce the death of infected cells to prevent virus transmission. ZBP1 can be activated and causes necrocytosis without viral infection, which is associated with nuclear Z-form nucleic acids. ZBP1 can be detected 42 kDa, 55 kDa, 68 kDa isoforms (PMID: 37012234, PMID: 9121465).