ZBP1 Rekombinanter Antikörper

ZBP1 Rekombinant Antikörper für IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

241582E2

Kat-Nr. : 84396-2-PBS

Synonyme

241582E2, C20orf183, DAI, DLM 1, DLM1

---

• 

  Sandwich ELISA standard curve of MP01267-1

  Sandwich ELISA standard curve of MP01267-1, Human ZBP1 Recombinant Matched Antibody Pair - PBS only. 84396-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag4119. 84396-2-PBS was HRP conjugated as the detection antibody. Range: 156-5000 pg/mL.

• 

  Sample test of MP01267-1

  The mean ZBP1 concentration was determined to be 7.1 ng/mL in HeLa cell extract based on a 2.8 mg/mL extract load, 15.4 ng/mL in Jurkat cell extract based on a 1.5 mg/mL extract load and 9.3 ng/mL in HL-60 cell extract based on a 1.4 mg/mL extract load.

• 

  IHC staining of human colon cancer using 84396-2-RR (same clone as 84396-2-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84396-2-RR (ZBP1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84396-2-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 84396-2-RR (same clone as 84396-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ZBP1 antibody (84396-2-RR, Clone: 241582E2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84396-2-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01267-1

  Cytometric bead array standard curve of MP01267-1, ZBP1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84396-1-PBS. Detection antibody: 84396-2-PBS. Standard: Ag4119. Range: 0.781-100 ng/mL.

• 

  Affinity and Kinetic Characterization of 84396-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84396-2-RR against Human ZBP1 were performed. The affinity constant is 5.12 nM.

---

Geprüfte Anwendungen

Produktinformation

84396-2-PBS bindet in IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test ZBP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: ZBP1 fusion protein Ag4119
• Vollständiger Name: Z-DNA binding protein 1
• Berechnetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC028218
• Gene symbol: ZBP1
• Gene ID (NCBI): 81030
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ZBP1(Z-DNA-binding protein 1), also named as DAI or DLM-1, is a nucleic acid sensor with some isoforms. It has been reported that ZBP1 can sense the viral nucleic acids of viruses and induce the death of infected cells to prevent virus transmission. ZBP1 can be activated and causes necrocytosis without viral infection, which is associated with nuclear Z-form nucleic acids. ZBP1 can be detected 42 kDa, 55 kDa, 68 kDa isoforms (PMID: 37012234, PMID: 9121465).